Effect of long-term storage and use on the properties of reversed-phase liquid chromatographic columns

Erik Haghedooren, Edit Farkas, Ágnes Kerner, Sanja Dragovic, B. Noszál, Jos Hoogmartens, Erwin Adams

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In order to study column deterioration as a result of long-term storage and/or usage in liquid chromatography analyses, 55 pairs (same batch) of different commercial reversed-phase C18 columns were examined using an already existing column characterisation system. After initial testing, one column was stored and the other was used to analyse different pharmaceuticals. All columns were characterized by four chromatographic parameters reflecting hydrophobicity, silanol activity, metal impurity and steric selectivity at the beginning and at the end of the test. An F-value was calculated to express the change of column properties with one single number. After performing analyses, higher F-values were obtained as compared to the non-used, stored columns. Although the time during which the columns were used to perform analyses was relatively short, an obvious influence was noticed, mainly resulting from small changes in silanol activity and hydrophobicity. Most of the affected columns have no endcapping and/or no base deactivation, making them more vulnerable for degradation, resulting in higher silanol activity and faster ageing. This effect is observed less with columns equipped with polar-embedded groups and/or polar endcapping, protecting the column by blocking the silanol groups and attracting a shielding water layer. Also columns with higher coverages and bulky or long chains show more resistance towards degradation.

Original languageEnglish
Pages (from-to)172-182
Number of pages11
JournalTalanta
Volume76
Issue number1
DOIs
Publication statusPublished - Jun 30 2008

Fingerprint

Liquids
Hydrophobicity
Hydrophobic and Hydrophilic Interactions
Degradation
Liquid chromatography
Liquid Chromatography
Shielding
Deterioration
Aging of materials
Metals
Impurities
silanol
Water
Testing
Pharmaceutical Preparations

Keywords

  • Chromatographic tests
  • Column characterisation
  • Column classification
  • Long-term usage
  • Reversed-phase liquid chromatography
  • Storage

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Haghedooren, E., Farkas, E., Kerner, Á., Dragovic, S., Noszál, B., Hoogmartens, J., & Adams, E. (2008). Effect of long-term storage and use on the properties of reversed-phase liquid chromatographic columns. Talanta, 76(1), 172-182. https://doi.org/10.1016/j.talanta.2008.02.020

Effect of long-term storage and use on the properties of reversed-phase liquid chromatographic columns. / Haghedooren, Erik; Farkas, Edit; Kerner, Ágnes; Dragovic, Sanja; Noszál, B.; Hoogmartens, Jos; Adams, Erwin.

In: Talanta, Vol. 76, No. 1, 30.06.2008, p. 172-182.

Research output: Contribution to journalArticle

Haghedooren, E, Farkas, E, Kerner, Á, Dragovic, S, Noszál, B, Hoogmartens, J & Adams, E 2008, 'Effect of long-term storage and use on the properties of reversed-phase liquid chromatographic columns', Talanta, vol. 76, no. 1, pp. 172-182. https://doi.org/10.1016/j.talanta.2008.02.020
Haghedooren, Erik ; Farkas, Edit ; Kerner, Ágnes ; Dragovic, Sanja ; Noszál, B. ; Hoogmartens, Jos ; Adams, Erwin. / Effect of long-term storage and use on the properties of reversed-phase liquid chromatographic columns. In: Talanta. 2008 ; Vol. 76, No. 1. pp. 172-182.
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