Effect of linear polymer additives on the electroosmotic characteristics of agarose gels in ultrathin-layer electrophoresis

Timea Lengyel, A. Guttman

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Electroosmotic properties of agarose gels with low, medium, high and super high electroendosmosis (EEO) were evaluated based on the apparent electric field mediated mobility of a neutral, fluorescent marker under constant field strength using ultrathin-layer separation configuration. Electroosmotic flow mobility values were measured in different gel concentrations and also in the absence and the presence of various linear polymer additives. Under ultrathin-layer separation conditions, a slight decrease in electroosmotic flow mobility was observed with increasing agarose gel concentration of 1 to 3% for all agarose gels investigated. When linear polymer additives, such as linear polyacrylamide, hydroxyethyl cellulose or polyethylene oxide were added to 1% low electroendosmosis agarose gel, significant reduction of the electroosmotic flow properties were observed with increasing additive concentration. Effect of the intrinsic electroosmotic properties of the various electroendosmosis agaroses on the apparent mobilities and separation performance of double-stranded DNA fragments during automated ultrathin-layer agarose gel electrophoresis was also investigated. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)511-518
Number of pages8
JournalJournal of Chromatography A
Volume853
Issue number1-2
DOIs
Publication statusPublished - Aug 20 1999

Fingerprint

Electrophoresis
Sepharose
Electroosmosis
Polymers
Gels
Agar Gel Electrophoresis
Cellulose
Electric fields
DNA

Keywords

  • Agarose gels
  • DNA
  • Electroosmotic flow
  • Ultrathin-layer electrophoresis

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Effect of linear polymer additives on the electroosmotic characteristics of agarose gels in ultrathin-layer electrophoresis. / Lengyel, Timea; Guttman, A.

In: Journal of Chromatography A, Vol. 853, No. 1-2, 20.08.1999, p. 511-518.

Research output: Contribution to journalArticle

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