Effect of lanthanum on red blood cell deformability

Tamas Alexy, N. Németh, Rosalinda B. Wenby, Rupert M. Bauersachs, Oguz K. Baskurt, Herbert J. Meiselman

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Prior reports describing the effects of lanthanum (La3+) on red blood cells (RBC) have focused on the effects of this lanthanide on cell fusion or on membrane characteristics (e.g., ion movement across membrane, membrane protein aggregation); the present study explores its rheological and biophysical effects. Normal human RBC were exposed to La3+ levels up to 200 μM then tested for: (1) cellular deformability using a laser-based ektacytometer and an optical-based rheoscope; (2) membrane viscoelastic behavior via micropipettes; (3) surface charge via micro electrophoresis. La 3+ concentrations of 12.5 to 200 μM caused dose-dependent decreases of deformability that were greatest at low stresses: these rheological changes were completely reversible upon removing La3+ from the media either by washing with La3+-free buffer or by suspending La 3+-exposed cells in La3+-free media (i.e., viscous dextran solution). Both membrane shear elastic modulus and membrane surface viscosity were increased by 25-30% at 100 or 200 μM. As expected, La3+ decreased RBC electrophoretic mobility (EPM), with EPM inversely but not linearly associated with deformability; changes of EPM were also completely reversible. These results thus indicate novel aspects of RBC cellular and membrane rheological behavior yet raise questions regarding specific mechanisms responsible for La3+-induced alterations.

Original languageEnglish
Pages (from-to)361-373
Number of pages13
JournalBiorheology
Volume44
Issue number5-6
Publication statusPublished - 2007

Fingerprint

Lanthanum
Erythrocytes
Membranes
Lanthanoid Series Elements
Cell Fusion
Elastic Modulus
Dextrans
Viscosity
Electrophoresis
Buffers
Membrane Proteins
Lasers
Cell Membrane
Ions

Keywords

  • Deformability
  • Electrophoresis
  • Lanthanum
  • Membrane
  • Red blood cell

ASJC Scopus subject areas

  • Physiology (medical)
  • Physiology

Cite this

Alexy, T., Németh, N., Wenby, R. B., Bauersachs, R. M., Baskurt, O. K., & Meiselman, H. J. (2007). Effect of lanthanum on red blood cell deformability. Biorheology, 44(5-6), 361-373.

Effect of lanthanum on red blood cell deformability. / Alexy, Tamas; Németh, N.; Wenby, Rosalinda B.; Bauersachs, Rupert M.; Baskurt, Oguz K.; Meiselman, Herbert J.

In: Biorheology, Vol. 44, No. 5-6, 2007, p. 361-373.

Research output: Contribution to journalArticle

Alexy, T, Németh, N, Wenby, RB, Bauersachs, RM, Baskurt, OK & Meiselman, HJ 2007, 'Effect of lanthanum on red blood cell deformability', Biorheology, vol. 44, no. 5-6, pp. 361-373.
Alexy T, Németh N, Wenby RB, Bauersachs RM, Baskurt OK, Meiselman HJ. Effect of lanthanum on red blood cell deformability. Biorheology. 2007;44(5-6):361-373.
Alexy, Tamas ; Németh, N. ; Wenby, Rosalinda B. ; Bauersachs, Rupert M. ; Baskurt, Oguz K. ; Meiselman, Herbert J. / Effect of lanthanum on red blood cell deformability. In: Biorheology. 2007 ; Vol. 44, No. 5-6. pp. 361-373.
@article{7601e81a68414492bac640ea6423241a,
title = "Effect of lanthanum on red blood cell deformability",
abstract = "Prior reports describing the effects of lanthanum (La3+) on red blood cells (RBC) have focused on the effects of this lanthanide on cell fusion or on membrane characteristics (e.g., ion movement across membrane, membrane protein aggregation); the present study explores its rheological and biophysical effects. Normal human RBC were exposed to La3+ levels up to 200 μM then tested for: (1) cellular deformability using a laser-based ektacytometer and an optical-based rheoscope; (2) membrane viscoelastic behavior via micropipettes; (3) surface charge via micro electrophoresis. La 3+ concentrations of 12.5 to 200 μM caused dose-dependent decreases of deformability that were greatest at low stresses: these rheological changes were completely reversible upon removing La3+ from the media either by washing with La3+-free buffer or by suspending La 3+-exposed cells in La3+-free media (i.e., viscous dextran solution). Both membrane shear elastic modulus and membrane surface viscosity were increased by 25-30{\%} at 100 or 200 μM. As expected, La3+ decreased RBC electrophoretic mobility (EPM), with EPM inversely but not linearly associated with deformability; changes of EPM were also completely reversible. These results thus indicate novel aspects of RBC cellular and membrane rheological behavior yet raise questions regarding specific mechanisms responsible for La3+-induced alterations.",
keywords = "Deformability, Electrophoresis, Lanthanum, Membrane, Red blood cell",
author = "Tamas Alexy and N. N{\'e}meth and Wenby, {Rosalinda B.} and Bauersachs, {Rupert M.} and Baskurt, {Oguz K.} and Meiselman, {Herbert J.}",
year = "2007",
language = "English",
volume = "44",
pages = "361--373",
journal = "Biorheology",
issn = "0006-355X",
publisher = "IOS Press",
number = "5-6",

}

TY - JOUR

T1 - Effect of lanthanum on red blood cell deformability

AU - Alexy, Tamas

AU - Németh, N.

AU - Wenby, Rosalinda B.

AU - Bauersachs, Rupert M.

AU - Baskurt, Oguz K.

AU - Meiselman, Herbert J.

PY - 2007

Y1 - 2007

N2 - Prior reports describing the effects of lanthanum (La3+) on red blood cells (RBC) have focused on the effects of this lanthanide on cell fusion or on membrane characteristics (e.g., ion movement across membrane, membrane protein aggregation); the present study explores its rheological and biophysical effects. Normal human RBC were exposed to La3+ levels up to 200 μM then tested for: (1) cellular deformability using a laser-based ektacytometer and an optical-based rheoscope; (2) membrane viscoelastic behavior via micropipettes; (3) surface charge via micro electrophoresis. La 3+ concentrations of 12.5 to 200 μM caused dose-dependent decreases of deformability that were greatest at low stresses: these rheological changes were completely reversible upon removing La3+ from the media either by washing with La3+-free buffer or by suspending La 3+-exposed cells in La3+-free media (i.e., viscous dextran solution). Both membrane shear elastic modulus and membrane surface viscosity were increased by 25-30% at 100 or 200 μM. As expected, La3+ decreased RBC electrophoretic mobility (EPM), with EPM inversely but not linearly associated with deformability; changes of EPM were also completely reversible. These results thus indicate novel aspects of RBC cellular and membrane rheological behavior yet raise questions regarding specific mechanisms responsible for La3+-induced alterations.

AB - Prior reports describing the effects of lanthanum (La3+) on red blood cells (RBC) have focused on the effects of this lanthanide on cell fusion or on membrane characteristics (e.g., ion movement across membrane, membrane protein aggregation); the present study explores its rheological and biophysical effects. Normal human RBC were exposed to La3+ levels up to 200 μM then tested for: (1) cellular deformability using a laser-based ektacytometer and an optical-based rheoscope; (2) membrane viscoelastic behavior via micropipettes; (3) surface charge via micro electrophoresis. La 3+ concentrations of 12.5 to 200 μM caused dose-dependent decreases of deformability that were greatest at low stresses: these rheological changes were completely reversible upon removing La3+ from the media either by washing with La3+-free buffer or by suspending La 3+-exposed cells in La3+-free media (i.e., viscous dextran solution). Both membrane shear elastic modulus and membrane surface viscosity were increased by 25-30% at 100 or 200 μM. As expected, La3+ decreased RBC electrophoretic mobility (EPM), with EPM inversely but not linearly associated with deformability; changes of EPM were also completely reversible. These results thus indicate novel aspects of RBC cellular and membrane rheological behavior yet raise questions regarding specific mechanisms responsible for La3+-induced alterations.

KW - Deformability

KW - Electrophoresis

KW - Lanthanum

KW - Membrane

KW - Red blood cell

UR - http://www.scopus.com/inward/record.url?scp=58149112921&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149112921&partnerID=8YFLogxK

M3 - Article

C2 - 18401075

AN - SCOPUS:58149112921

VL - 44

SP - 361

EP - 373

JO - Biorheology

JF - Biorheology

SN - 0006-355X

IS - 5-6

ER -