Az ischaemiás posztkondicionálás hatása az oxidatív stresszre és a szöveti struktúrára vékonybél meleg ischaemiás és autotranszplantációs modellekben.

Translated title of the contribution: [Effect of ischemic postconditioning on oxidative stress and structural tissue changes in intestinal warm ischemic and autotransplantation models].

Klára Nedvig, Edina Völgyi, György Wéber, Erzsébet Röth, Andrea Ferencz

Research output: Contribution to journalArticle

Abstract

Our study investigated the effect of ischemic postconditioning (IPO) in intestinal warm ischemia/reperfusion (I/R) and autotransplantation models. Warm ischemia was performed by occlusion of superior mesenteric artery for 1, 3 and 6 hours in white domestic pigs (n = 15). Prior to 3 hours reperfusion the intestine was postconditioned by 3 cycles of 30-seconds ischemia and 30-seconds reperfusion (IPO protocol). In the cold ischemia group (n = 15) the bowel was preserved in University of Wisconsin solution for 1, 3, and 6 hours. Prior to 3 hours reperfusion IPO protocol was applied, too. Tissue samples were collected after laparotomy (control) and at the end of the reperfusion periods. As far as oxidative stress markers, malondialdehyde and reduced glutathione (GSH) levels and superoxide dismutase (SOD) activity were determined. Tissue damage was evaluated by qualitative (Park-classification) and quantitative (Scion Image) methods. As regards oxidative stress parameters, lipidperoxidation decreased and the protective effect of endogenous antioxidants (GSH, SOD) retained significantly by IPO procedure at the end of reperfusion. Tissue injury correlated significantly by the duration of warm ischemia and cold preservation. Quantitative analysis demonstrated that IPO ameliorated tissue injury in each group (p < 0.05). IPO significantly attenuated intestinal oxidative stress and morphological damages in warm and cold I/R models.

Original languageHungarian
Pages (from-to)294-300
Number of pages7
JournalMagyar sebészet
Volume64
Issue number6
Publication statusPublished - Dec 2011

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ASJC Scopus subject areas

  • Medicine(all)

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