Effect of ischaemia on the enzyme activity of the hepatic tissue

P. Kupcsulik, K. Stekker, M. Németh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The lactate and pyruvate levels, as well as acid and alkaline phosphatase, lactate dehydrogenase, glucose-6-phosphate dehydrogenase, glutaminic acid-oxalacetic acid transaminase and aldolase levels of rat liver homogenizates were measured at 24°C and 38°C during 120 min ischaemia from 0 to the 120th min. With the exception of transaminase and aldolase, the other enzymes were also enzyme-histochemically studied. The early lesions of the liver can be detected, both the quantitative laboratory tests and enzyme histochemical studies. The deviations from normal, observed at 24°C between the 60th and 100th min, and at 38°C between the 30th and 60th min, might be signs of irreversible lesions. Fractionated study of the liver homogenizate improves the assessability of enzyme determinations. In the course of "warm" ischaemia, the liver lysosomal lesions are early symptoms. Parallel to the breakdown of aerobic glycolysis lactic acid, fermentation, and an active pentose phosphate cycle can be detected. Quantitative testing of the liver homogenizate and enzyme histochemical observation of the hepatic tissue, might be a suitable method for the assessment of ischaemic liver lesions.

Original languageEnglish
Pages (from-to)259-270
Number of pages12
JournalResearch in Experimental Medicine
Volume170
Issue number3
DOIs
Publication statusPublished - Oct 1977

Fingerprint

Ischemia
Liver
Enzymes
Fructose-Bisphosphate Aldolase
Transaminases
Lactic Acid
Glutamates
Pentoses
Oxaloacetic Acid
Warm Ischemia
Glucosephosphate Dehydrogenase
Glycolysis
Acid Phosphatase
Pyruvic Acid
L-Lactate Dehydrogenase
Fermentation
Alkaline Phosphatase
Phosphates
Observation

Keywords

  • Enzymes
  • Histochemistry
  • Ischaemia
  • Liver
  • Preservation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effect of ischaemia on the enzyme activity of the hepatic tissue. / Kupcsulik, P.; Stekker, K.; Németh, M.

In: Research in Experimental Medicine, Vol. 170, No. 3, 10.1977, p. 259-270.

Research output: Contribution to journalArticle

Kupcsulik, P. ; Stekker, K. ; Németh, M. / Effect of ischaemia on the enzyme activity of the hepatic tissue. In: Research in Experimental Medicine. 1977 ; Vol. 170, No. 3. pp. 259-270.
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