Effect of iron oxide loading on magnetoferritin structure in solution as revealed by SAXS and SANS

L. Melníková, V. I. Petrenko, M. V. Avdeev, V. M. Garamus, L. Almásy, O. I. Ivankov, L. A. Bulavin, Z. Mitróová, P. Kopčanský

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Synthetic biological macromolecule of magnetoferritin containing an iron oxide core inside a protein shell (apoferritin) is prepared with different content of iron. Its structure in aqueous solution is analysed by small-angle synchrotron X-ray (SAXS) and neutron (SANS) scattering. The loading factor (LF) defined as the average number of iron atoms per protein is varied up to LF. =. 800. With an increase of the LF, the scattering curves exhibit a relative increase in the total scattered intensity, a partial smearing and a shift of the match point in the SANS contrast variation data. The analysis shows an increase in the polydispersity of the proteins and a corresponding effective increase in the relative content of magnetic material against the protein moiety of the shell with the LF growth. At LFs above ~150, the apoferritin shell undergoes structural changes, which is strongly indicative of the fact that the shell stability is affected by iron oxide presence.

Original languageEnglish
Pages (from-to)82-88
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume123
DOIs
Publication statusPublished - Nov 1 2014

Fingerprint

Iron oxides
iron oxides
Apoferritins
proteins
Proteins
shell stability
Iron
iron
Synchrotrons
Magnetic materials
Neutrons
Polydispersity
Neutron scattering
magnetic materials
Macromolecules
macromolecules
Intercellular Signaling Peptides and Proteins
synchrotrons
neutron scattering
X-Rays

Keywords

  • Apoferritin
  • Biorelevant magnetic nanoparticles
  • Contrast variation
  • Magnetoferritin
  • Small-angle neutron scattering
  • Small-angle X-ray scattering

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Effect of iron oxide loading on magnetoferritin structure in solution as revealed by SAXS and SANS. / Melníková, L.; Petrenko, V. I.; Avdeev, M. V.; Garamus, V. M.; Almásy, L.; Ivankov, O. I.; Bulavin, L. A.; Mitróová, Z.; Kopčanský, P.

In: Colloids and Surfaces B: Biointerfaces, Vol. 123, 01.11.2014, p. 82-88.

Research output: Contribution to journalArticle

Melníková, L, Petrenko, VI, Avdeev, MV, Garamus, VM, Almásy, L, Ivankov, OI, Bulavin, LA, Mitróová, Z & Kopčanský, P 2014, 'Effect of iron oxide loading on magnetoferritin structure in solution as revealed by SAXS and SANS', Colloids and Surfaces B: Biointerfaces, vol. 123, pp. 82-88. https://doi.org/10.1016/j.colsurfb.2014.08.032
Melníková, L. ; Petrenko, V. I. ; Avdeev, M. V. ; Garamus, V. M. ; Almásy, L. ; Ivankov, O. I. ; Bulavin, L. A. ; Mitróová, Z. ; Kopčanský, P. / Effect of iron oxide loading on magnetoferritin structure in solution as revealed by SAXS and SANS. In: Colloids and Surfaces B: Biointerfaces. 2014 ; Vol. 123. pp. 82-88.
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