Effect of intrathecal capsaicin analogues on the immunofluorescence of peptides and serotonin in the dorsal horn in rats

P. E. Micevych, T. L. Yaksh, J. Szolcsányi

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Abstract

The intrathecal administration of capsaicin, a homovanillylamide derivative, has been demonstrated to cause analgesia in response to thermal stimuli. This analgesia has been correlated with a profound depletion of spinal substance P, a putative primary afferent transmitter. We studied the effects of capsaicin, a series of capsaicin analogues, piperine and kainic acid on the immunohistochemical staining of substance P, cholecystokinin, somatostatin, methionine-enkephalin and serotonin. Capsaicin and an analogue 1-nonenoyl-vanillylamide significantly elevated the tail flick latency and when the spinal cords of the rats were analyzed immunohistochemically, a profound depletion of substance P and cholecystokinin was observed. The spinal somatostatin-immunoreactivity of these rats was slightly reduced. Piperine also depleted substance P and reduced somatostatin staining but did not alter the staining intensity or density of cholecystokinin, methionine-enkephalin or serotonin. Kainate-depleted methionine-enkephalin but did not alter any other neuropeptides studied or serotonin. These results may indicate a link between capsaicin-induced analgesia and the concomitant depletion of cholecystokinin and substance P.

Original languageEnglish
Pages (from-to)123-131
Number of pages9
JournalNeuroscience
Volume8
Issue number1
DOIs
Publication statusPublished - 1983

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Capsaicin
Substance P
piperine
Cholecystokinin
Fluorescent Antibody Technique
Serotonin
Methionine Enkephalin
Peptides
Somatostatin
Analgesia
Kainic Acid
Staining and Labeling
Neuropeptides
Tail
Spinal Cord
Hot Temperature
Spinal Cord Dorsal Horn

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Effect of intrathecal capsaicin analogues on the immunofluorescence of peptides and serotonin in the dorsal horn in rats. / Micevych, P. E.; Yaksh, T. L.; Szolcsányi, J.

In: Neuroscience, Vol. 8, No. 1, 1983, p. 123-131.

Research output: Contribution to journalArticle

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