Effect of iloprost on reperfusion-induced arrhythmias and myocardial ion shifts in isolated rat hearts

Arpad Tosaki, Matyas Koltai, Monique Paubert-Braquet

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Isolated hearts excised from normotensive (NT) and spontaneously hypertensive (SH) rats subjected to transient normothermic global ischemia were used to study the effect of chronic treatment with iloprost on reperfusion-induced arrhythmias and myocardiat ion shifts. After 30 min of ischemia, iloprost given s.c. in doses of 10, 50, 100 and 200 μg/kg per day for 14 days reduced the incidence of reperfusion-induced ventricular fibrillation (VF) in isolated hearts from the control value of 91 to 83. 75, 50 (P < 0.05) and 25% (P < 0.01) respectively, in NT rats. In the SH groups, the incidence of VF was also reduced from 100 to 75, 58, 33 (P < 0.01) and 17% (P < 0.001), respectively, with 10, 50,100 and 200 (μg/kg per day of iloprost. A similar reduction was observed in the incidence of reperfusion-induced ventricular tachycardia (VT). Ischemia and reperfusion caused significant changes in myocardial ion contents, i.e. an increase in Na+ and Ca2+ and a decrease in K+ and Mg2+ concentrations. The myocardial water content was also increased in parallel to the Na+ gain. The effect of iloprost given s.c. in doses of 50 and 200 μg/kg per day for 14 days was also measured on myocardial ion contents after 15- or 30-min ischemia and 30-min ischemia plus 10-min reperfusion. The higher iloprost dose significantly reduced the myocardial Na+ Ca2+ and water gains and the loss of K+ induced by ischemia and reperfusion in the NT and SH groups, while the decrease in Mg2+ content was alleviated only in SH rats. The results suggest that long-term iloprost treatment reduces the incidence of reperfusion-induced VF and VT by preventing Na+, Ca2+ and water accumulation as well as K+ and Mg2+ loss from myocardial tissue.

Original languageEnglish
Pages (from-to)69-81
Number of pages13
JournalEuropean Journal of Pharmacology
Volume191
Issue number1
DOIs
Publication statusPublished - Nov 20 1990

    Fingerprint

Keywords

  • Arrhythmias
  • Heart (rat)
  • Myocardial K, Myocardial Ca
  • Myocardial Mg
  • Myocardial Na
  • Reperfusion

ASJC Scopus subject areas

  • Pharmacology

Cite this