Effect of IBD sera on expression of inducible and endothelial nitric oxide synthase in human umbilical vein endothelial cells

K. Palatka, Z. Serfőző, Zoltán Veréb, Róbert Bátori, Beáta Lontay, Zoltán Hargitay, Z. Nemes, Miklós Urvardy, F. Erdődi, I. Altorjay

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Aim: To study the expression of endothelial and inducible nitric oxide synthases (eNOS and iNOS) and their role in inflammatory bowel disease (IBD). Methods: We examined the effect of sera obtained from patients with active Crohn's disease (CD) and ulcerative colitis (UC) on the function and viability of human umbilical vein endothelial cells (HUVEC). HUVECs were cultured for 0-48 h in the presence of a medium containing pooled serum of healthy controls, or serum from patients with active CD or UC. Expression of eNOS and iNOS was visualized by immunofluorescence, and quantified by the densitometry of Western blots. Proliferation activity was assessed by computerized image analyses of Ki-67 immunoreactive cells, and also tested in the presence of the NOS inhibitor, 10-4 mol/ L L-NAME. Apoptosis and necrosis was examined by the annexin-V-biotin method and by propidium iodide staining, respectively. Results: In HUVE C immediately after exposure to UC, serum eNOS was markedly induced, reaching a peak at 12 h. In contrast, a decrease in eNOS was observed after incubation with CD sera and the eNOS level was minimal at 20 h compared to control (18%±16% vs 23%±15% P

Original languageEnglish
Pages (from-to)1730-1738
Number of pages9
JournalWorld Journal of Gastroenterology
Volume12
Issue number11
Publication statusPublished - Mar 21 2006

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Nitric Oxide Synthase Type III
Human Umbilical Vein Endothelial Cells
Nitric Oxide Synthase Type II
Inflammatory Bowel Diseases
Ulcerative Colitis
Crohn Disease
Serum
Propidium
Densitometry
Annexin A5
NG-Nitroarginine Methyl Ester
Biotin
Fluorescent Antibody Technique
Necrosis
Western Blotting
Apoptosis
Staining and Labeling

Keywords

  • Crohn's disease
  • Human umbilical vein endothelial cells
  • Inflammatory bowel disease
  • Nitric oxide synthase
  • Ulcerative colitis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Effect of IBD sera on expression of inducible and endothelial nitric oxide synthase in human umbilical vein endothelial cells. / Palatka, K.; Serfőző, Z.; Veréb, Zoltán; Bátori, Róbert; Lontay, Beáta; Hargitay, Zoltán; Nemes, Z.; Urvardy, Miklós; Erdődi, F.; Altorjay, I.

In: World Journal of Gastroenterology, Vol. 12, No. 11, 21.03.2006, p. 1730-1738.

Research output: Contribution to journalArticle

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AU - Veréb, Zoltán

AU - Bátori, Róbert

AU - Lontay, Beáta

AU - Hargitay, Zoltán

AU - Nemes, Z.

AU - Urvardy, Miklós

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AU - Altorjay, I.

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