Efecto de la hiperlipemia sobre la blastogénesis inducida por mitógenos, en linfocitos aislados de bazos de ratón.

Translated title of the contribution: Effect of hyperlipemia on mitogen-induced blastogenesis in lymphocytes isolated from the rat spleen

A. Somogyi, R. González-Cabello, E. Szondy, A. Blazovics, P. Gergely, J. Fehér

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Lipoproteins suppress several immune functions (mitogen-induced lymphoblast transformation, macrophage functions). Low density lipoprotein (LDL) is responsible for most of these effects. Diet may cause an elevation in LDL level. Therefore, we investigated the effect of cholesterol-rich diet upon the mitogen response of rats. The cholesterol and LDL-cholesterol level of rats was elevated after 8 days of diet. The blastogenic response of spleen cells separated by Ficoll-Paque gradient decreased. The response to phytomitogens 1, and 10 micrograms/ml phytohaemagglutinin (PHA) and 10 micrograms/ml concanavalin A (Con A) was measured by thymidine incorporation after 3 days of culture. The decrease of blastogenic response was more pronounced at the higher doses (10 micrograms/ml PHA, and 10 micrograms/ml Con A). For the lymphocyte cultures that did not contain autologous (rat) serum, we suggest that suppressed response was caused by an alteration of either in the membrane or in the metabolism of cells participating in the mitogen response. The decreased amount of LDL receptors and a derangement in intracellular cholesterol biosynthesis are supposed. The clinical implications of the immunosuppressive effect of dietary cholesterol is discussed.

Translated title of the contributionEffect of hyperlipemia on mitogen-induced blastogenesis in lymphocytes isolated from the rat spleen
Original languageSpanish
Pages (from-to)89-92
Number of pages4
JournalAllergologia et immunopathologia
Volume15
Issue number2
Publication statusPublished - Mar 1 1987

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pulmonary and Respiratory Medicine

Fingerprint Dive into the research topics of 'Effect of hyperlipemia on mitogen-induced blastogenesis in lymphocytes isolated from the rat spleen'. Together they form a unique fingerprint.

  • Cite this