Effect of human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models

Duangjai Boonkusol, A. Dinnyés, Tassanee Faisaikarm, Parisatcha Sangsuwan, Nathnapith Pratipnatalang, Mayurachat Sa-Ardrit, Kulnasan Saikhun, Saovaros Svasti, Jim Vadolas, Pranee Winichagoon, Suthat Fucharoen, Yindee Kitiyanant

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The purpose of the present study was to investigate the efficiency of embryo cryopreservation for four transgenic (TG) thalassaemic mouse strains, which is a key element of the ongoing gene banking efforts for these highvalue animals. Heterozygous TG embryos were produced by breeding four lines of TG males to wild-type (WT) females (C57BL/6J). Intact two-cell embryos were cryopreserved by vitrification in straws using 35% ethylene glycol. Survival rates of cryopreserved embryos ranged between 91.1% (102/112) and 93.6% (176/188) without significant differences between the lines. In contrast, the paternal line had a significant effect on the development of these embryos to the blastocyst stage, which ranged from 50.6% (92/182) to 77.5% (79/102). This effect was also noted following embryo transfers, with implantation rates varying from 17.3% (19/110) to 78.1% (35/45). The results demonstrate that the in vivo developmental potential is significantly influenced byTG line and reveal a specific line effect on cryosurvival. All bacterial artificial chromosome transgenic fetuses developed from vitrified-warmed embryos showed expression of the human β-globin transgene. In conclusion, the present study shows a strongTG line effect on developmental competence following cryopreservation and the vitrification method was successful to bank the human β-globin TG-expressing mouse strains.

Original languageEnglish
Pages (from-to)788-795
Number of pages8
JournalReproduction, Fertility and Development
Volume22
Issue number5
DOIs
Publication statusPublished - 2010

Fingerprint

Bacterial Artificial Chromosomes
Gene Transfer Techniques
bacterial artificial chromosomes
Globins
Cryopreservation
cryopreservation
embryo (animal)
Embryonic Structures
animal models
genetically modified organisms
Vitrification
vitrification
Transgenic Mice
banking
Ethylene Glycol
Embryo Transfer
ethylene glycol
mice
Blastocyst
embryo transfer

Keywords

  • Embryo banking
  • Thalassaemia transgenic

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology
  • Reproductive Medicine
  • Animal Science and Zoology

Cite this

Effect of human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models. / Boonkusol, Duangjai; Dinnyés, A.; Faisaikarm, Tassanee; Sangsuwan, Parisatcha; Pratipnatalang, Nathnapith; Sa-Ardrit, Mayurachat; Saikhun, Kulnasan; Svasti, Saovaros; Vadolas, Jim; Winichagoon, Pranee; Fucharoen, Suthat; Kitiyanant, Yindee.

In: Reproduction, Fertility and Development, Vol. 22, No. 5, 2010, p. 788-795.

Research output: Contribution to journalArticle

Boonkusol, D, Dinnyés, A, Faisaikarm, T, Sangsuwan, P, Pratipnatalang, N, Sa-Ardrit, M, Saikhun, K, Svasti, S, Vadolas, J, Winichagoon, P, Fucharoen, S & Kitiyanant, Y 2010, 'Effect of human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models', Reproduction, Fertility and Development, vol. 22, no. 5, pp. 788-795. https://doi.org/10.1071/RD09128
Boonkusol, Duangjai ; Dinnyés, A. ; Faisaikarm, Tassanee ; Sangsuwan, Parisatcha ; Pratipnatalang, Nathnapith ; Sa-Ardrit, Mayurachat ; Saikhun, Kulnasan ; Svasti, Saovaros ; Vadolas, Jim ; Winichagoon, Pranee ; Fucharoen, Suthat ; Kitiyanant, Yindee. / Effect of human β-globin bacterial artificial chromosome transgenesis on embryo cryopreservation in mouse models. In: Reproduction, Fertility and Development. 2010 ; Vol. 22, No. 5. pp. 788-795.
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AU - Sa-Ardrit, Mayurachat

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AU - Svasti, Saovaros

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