Effect of histone deacetylase inhibitor trichostatin a (TSA) on the microtubular system of Tetrahymena

P. Kovács, E. Pállinger, G. Csaba

Research output: Contribution to journalArticle

Abstract

Histone deacetylases can also influence acetylation of tubulin. In the present experiments, after 60 min of 10 μM trichostatin (TSA) treatment the structure and amount of tubulin and acetylated-tubulin were studied immunocytochemically, by using confocal microscopy and flow cytometry. In TSA-treated Tetrahymena cells deep fibres were never labeled with antibody to acetylated tubulin. Flow cytometry with anti acetylated-tubulin antibody demonstrated that in the control cell populations there were weaker and stronger labelled parts. After TSA treatment in the weaker labeled part the cell number decreased, and in the stronger labeled part increased significantly: this means that after the histone deacetylase inhibitor TSA treatment the amount of acetylated-tubulin in numerous Tetrahymena cells is significantly elevated. Labeling with anti-tubulin antibody was not changed significantly. On the basis of these results we postulate that histone deacetylase also in Tetrahymena influences the acetylation of tubulin, and this enzyme is sensitive to TSA treatments.

Original languageEnglish
Pages (from-to)381-387
Number of pages7
JournalActa Biologica Hungarica
Volume58
Issue number4
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Tetrahymena
histone deacetylase
Histone Deacetylase Inhibitors
Tubulin
tubulin
antibody
inhibitor
flow cytometry
Acetylation
Histone Deacetylases
Flow cytometry
microscopy
acetylation
enzyme
antibodies
Antibodies
Flow Cytometry
cells
effect
Confocal microscopy

Keywords

  • Histone deacetylase
  • Tetrahymena
  • Trichostatin A
  • Tubulin-acetylation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Effect of histone deacetylase inhibitor trichostatin a (TSA) on the microtubular system of Tetrahymena. / Kovács, P.; Pállinger, E.; Csaba, G.

In: Acta Biologica Hungarica, Vol. 58, No. 4, 12.2007, p. 381-387.

Research output: Contribution to journalArticle

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