Effect of hexavalent chromium on eukaryotic plasma membrane studied by EPR spectroscopy

J. Belágyi, Maya Pas, Peter Raspor, M. Pesti, T. Páli

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The effect of Cr(VI) anion on an ergosterol-producing strain of eukaryotic yeast Candida albicans and its mutant with ergosterol-less membrane was studied with EPR spectroscopy. 5- and 14-doxyl stearic acid spin probes were used to label the protoplast membrane after removal of the cell wall. In control experiments, the mutant strain exhibited larger rigidity in the membrane than its parental strain. Addition of Cr(VI), at a minimum inhibitory concentration of 0.6 mM, increased the rotational mobility of the spin labels significantly and decreased the temperature of the structural changes in both strains, in the temperature range between 0 and 30°C. The ergosterol-less mutant, having a membrane composition with increased polyunsaturated fatty acid content, exhibited higher Cr(VI) sensitivity. Treatment of the membrane with Cr(VI) for 10 min already resulted in an increase in membrane fluidity. An EPR signal of Cr(V) was detected which reached maximum amplitude after 120 min of treatment with Cr(VI). Further chemical reduction of Cr(V) in the absence of extracellular Cr(VI) led to a lack of detectable paramagnetic chromium intermediates within 200 min. Copyright (C) 1999.

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalBBA - Biomembranes
Volume1421
Issue number1
DOIs
Publication statusPublished - Sep 21 1999

Fingerprint

Cell membranes
Paramagnetic resonance
Spectrum Analysis
Cell Membrane
Spectroscopy
Membranes
Ergosterol
Spin Labels
Membrane Fluidity
Temperature
Fluidity
Protoplasts
Candida
Microbial Sensitivity Tests
Chromium
Candida albicans
Unsaturated Fatty Acids
Rigidity
Yeast
Cell Wall

Keywords

  • Candida albicans
  • Chromium VI
  • Membrane fluidity
  • Spin label EPR

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Effect of hexavalent chromium on eukaryotic plasma membrane studied by EPR spectroscopy. / Belágyi, J.; Pas, Maya; Raspor, Peter; Pesti, M.; Páli, T.

In: BBA - Biomembranes, Vol. 1421, No. 1, 21.09.1999, p. 175-182.

Research output: Contribution to journalArticle

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