Effect of hemicholinium-3 on the release and net synthesis of acetylcholine in Auerbach's plexus of guinea pig ileum

G. T. Somogyi, E. Vízi, J. Knoll

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The amount of acetylcholine released from innervated longitudinal muscle of the guinea-pig ileum evoked by continuous high frequency stimulation reached a peak value of 952.3 pmol · g-1 followed by a gradual decrease and formation of a new equilibrium state (568.4 pmol · g-1· min-1), which probably corresponded to the rate of synthesis. Hemicholinium-3 at a concentration of 10-6m did not inhibit the instantaneous release of acetylcholine neither from the stimulated nor from the non-stimulated organ, but completely prevented the establishment of a new equilibrium state. Although 50-70% of the acetylcholine content was still present in the tissues treated with hemicholinium-3, both the stimulation-induced and resting release was abolished. It is concluded that this part of the acetylcholine content is not available for release. When calculating the net synthesis of acetylcholine in hemicholinium-3 treated organs, 37.1 nmol · g-1 acetylcholine was missing, i.e. the synthesis was not only blocked but decomposition of acetylcholine occurred. The amount of decomposed ('lost') acetylcholine was identical both in stimulated and non-stimulated strips. It is suggested that backward reaction of choline acetyltransferase is responsible for this phenomenon.

Original languageEnglish
Pages (from-to)791-796
Number of pages6
JournalNeuroscience
Volume2
Issue number5
DOIs
Publication statusPublished - 1977

Fingerprint

Hemicholinium 3
Myenteric Plexus
Ileum
Acetylcholine
Guinea Pigs
Choline O-Acetyltransferase
Muscles

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Effect of hemicholinium-3 on the release and net synthesis of acetylcholine in Auerbach's plexus of guinea pig ileum. / Somogyi, G. T.; Vízi, E.; Knoll, J.

In: Neuroscience, Vol. 2, No. 5, 1977, p. 791-796.

Research output: Contribution to journalArticle

@article{d084b2270a2a4e34b8bda2d916b0df4d,
title = "Effect of hemicholinium-3 on the release and net synthesis of acetylcholine in Auerbach's plexus of guinea pig ileum",
abstract = "The amount of acetylcholine released from innervated longitudinal muscle of the guinea-pig ileum evoked by continuous high frequency stimulation reached a peak value of 952.3 pmol · g-1 followed by a gradual decrease and formation of a new equilibrium state (568.4 pmol · g-1· min-1), which probably corresponded to the rate of synthesis. Hemicholinium-3 at a concentration of 10-6m did not inhibit the instantaneous release of acetylcholine neither from the stimulated nor from the non-stimulated organ, but completely prevented the establishment of a new equilibrium state. Although 50-70{\%} of the acetylcholine content was still present in the tissues treated with hemicholinium-3, both the stimulation-induced and resting release was abolished. It is concluded that this part of the acetylcholine content is not available for release. When calculating the net synthesis of acetylcholine in hemicholinium-3 treated organs, 37.1 nmol · g-1 acetylcholine was missing, i.e. the synthesis was not only blocked but decomposition of acetylcholine occurred. The amount of decomposed ('lost') acetylcholine was identical both in stimulated and non-stimulated strips. It is suggested that backward reaction of choline acetyltransferase is responsible for this phenomenon.",
author = "Somogyi, {G. T.} and E. V{\'i}zi and J. Knoll",
year = "1977",
doi = "10.1016/0306-4522(77)90032-X",
language = "English",
volume = "2",
pages = "791--796",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Effect of hemicholinium-3 on the release and net synthesis of acetylcholine in Auerbach's plexus of guinea pig ileum

AU - Somogyi, G. T.

AU - Vízi, E.

AU - Knoll, J.

PY - 1977

Y1 - 1977

N2 - The amount of acetylcholine released from innervated longitudinal muscle of the guinea-pig ileum evoked by continuous high frequency stimulation reached a peak value of 952.3 pmol · g-1 followed by a gradual decrease and formation of a new equilibrium state (568.4 pmol · g-1· min-1), which probably corresponded to the rate of synthesis. Hemicholinium-3 at a concentration of 10-6m did not inhibit the instantaneous release of acetylcholine neither from the stimulated nor from the non-stimulated organ, but completely prevented the establishment of a new equilibrium state. Although 50-70% of the acetylcholine content was still present in the tissues treated with hemicholinium-3, both the stimulation-induced and resting release was abolished. It is concluded that this part of the acetylcholine content is not available for release. When calculating the net synthesis of acetylcholine in hemicholinium-3 treated organs, 37.1 nmol · g-1 acetylcholine was missing, i.e. the synthesis was not only blocked but decomposition of acetylcholine occurred. The amount of decomposed ('lost') acetylcholine was identical both in stimulated and non-stimulated strips. It is suggested that backward reaction of choline acetyltransferase is responsible for this phenomenon.

AB - The amount of acetylcholine released from innervated longitudinal muscle of the guinea-pig ileum evoked by continuous high frequency stimulation reached a peak value of 952.3 pmol · g-1 followed by a gradual decrease and formation of a new equilibrium state (568.4 pmol · g-1· min-1), which probably corresponded to the rate of synthesis. Hemicholinium-3 at a concentration of 10-6m did not inhibit the instantaneous release of acetylcholine neither from the stimulated nor from the non-stimulated organ, but completely prevented the establishment of a new equilibrium state. Although 50-70% of the acetylcholine content was still present in the tissues treated with hemicholinium-3, both the stimulation-induced and resting release was abolished. It is concluded that this part of the acetylcholine content is not available for release. When calculating the net synthesis of acetylcholine in hemicholinium-3 treated organs, 37.1 nmol · g-1 acetylcholine was missing, i.e. the synthesis was not only blocked but decomposition of acetylcholine occurred. The amount of decomposed ('lost') acetylcholine was identical both in stimulated and non-stimulated strips. It is suggested that backward reaction of choline acetyltransferase is responsible for this phenomenon.

UR - http://www.scopus.com/inward/record.url?scp=0017649134&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017649134&partnerID=8YFLogxK

U2 - 10.1016/0306-4522(77)90032-X

DO - 10.1016/0306-4522(77)90032-X

M3 - Article

C2 - 593557

AN - SCOPUS:0017649134

VL - 2

SP - 791

EP - 796

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 5

ER -