Effect of Helicobacter pylori infection on epidermal growth factor receptor (EGFR) expression and cell proliferation of gastric epithelial mucosa: Correlation to macroscopic and microscopic diagnosis

Agnes Ruzsovics, Zsuzsa Unger, B. Molnár, L. Prónai, Z. Tulassay

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8 Citations (Scopus)

Abstract

Our aim was to compare the expression of EGFR and proliferative cell nuclear antigen (PCNA) in different histological and endoscopic diagnostic groups, in cases of Helicobacter pylori infection, in vivo. Paraffin embedded human gastric biopsy samples (86) were analysed by EGFR and PCNA immunohistochemistry and classified both on the basis of histology and endoscopic findings. In normal epithelia (NE), a positive correlation was found between PCNA and EGFR and in H. pylori-negative gastritis with and without intestinal metaplasia (P <0.01). On the other hand, a negative correlation was detected between the two immunohistochemical findings in H. pylori-associated gastritis with intestinal metaplasia (HPGIM) and in the atrophic gastritis (AG) group. In HPGIM the percentage of EGFR-positive cells was significantly lower (32.4 ± 30.4) when compared to either the NE (50.3 ± 23.7) or H. pylori-negative gastritis with intestinal metaplasia (HNGIM) (48.3 ± 23.7). In AG, EGFR was significantly lower when compared to the NE (P <0.05). Based on the endoscopic findings, a significant decrease of EGFR expression was found in gastric ulcer cases as compared to NE, gastritis or erosion cases (P <0.01). PCNA showed no significant alterations between the NE and gastritis, AG groups. The presence of H. pylori has an inverse effect on PCNA and EGFR expression in HPGIM.

Original languageEnglish
Pages (from-to)257-263
Number of pages7
JournalInternational Journal of Experimental Pathology
Volume83
Issue number5
DOIs
Publication statusPublished - Oct 2002

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Helicobacter Infections
Gastritis
Gastric Mucosa
Epidermal Growth Factor Receptor
Helicobacter pylori
Nuclear Antigens
Metaplasia
Cell Proliferation
Epithelium
Atrophic Gastritis
Stomach Ulcer
Paraffin
Stomach
Histology
Immunohistochemistry
Biopsy

Keywords

  • Endoscopic diagnosis
  • Epidermal growth factor receptor
  • Helicobacter pylori infection
  • Histological diagnosis
  • Intestinal metaplasia
  • Proliferative cell nuclear antigen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

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title = "Effect of Helicobacter pylori infection on epidermal growth factor receptor (EGFR) expression and cell proliferation of gastric epithelial mucosa: Correlation to macroscopic and microscopic diagnosis",
abstract = "Our aim was to compare the expression of EGFR and proliferative cell nuclear antigen (PCNA) in different histological and endoscopic diagnostic groups, in cases of Helicobacter pylori infection, in vivo. Paraffin embedded human gastric biopsy samples (86) were analysed by EGFR and PCNA immunohistochemistry and classified both on the basis of histology and endoscopic findings. In normal epithelia (NE), a positive correlation was found between PCNA and EGFR and in H. pylori-negative gastritis with and without intestinal metaplasia (P <0.01). On the other hand, a negative correlation was detected between the two immunohistochemical findings in H. pylori-associated gastritis with intestinal metaplasia (HPGIM) and in the atrophic gastritis (AG) group. In HPGIM the percentage of EGFR-positive cells was significantly lower (32.4 ± 30.4) when compared to either the NE (50.3 ± 23.7) or H. pylori-negative gastritis with intestinal metaplasia (HNGIM) (48.3 ± 23.7). In AG, EGFR was significantly lower when compared to the NE (P <0.05). Based on the endoscopic findings, a significant decrease of EGFR expression was found in gastric ulcer cases as compared to NE, gastritis or erosion cases (P <0.01). PCNA showed no significant alterations between the NE and gastritis, AG groups. The presence of H. pylori has an inverse effect on PCNA and EGFR expression in HPGIM.",
keywords = "Endoscopic diagnosis, Epidermal growth factor receptor, Helicobacter pylori infection, Histological diagnosis, Intestinal metaplasia, Proliferative cell nuclear antigen",
author = "Agnes Ruzsovics and Zsuzsa Unger and B. Moln{\'a}r and L. Pr{\'o}nai and Z. Tulassay",
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T1 - Effect of Helicobacter pylori infection on epidermal growth factor receptor (EGFR) expression and cell proliferation of gastric epithelial mucosa

T2 - Correlation to macroscopic and microscopic diagnosis

AU - Ruzsovics, Agnes

AU - Unger, Zsuzsa

AU - Molnár, B.

AU - Prónai, L.

AU - Tulassay, Z.

PY - 2002/10

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N2 - Our aim was to compare the expression of EGFR and proliferative cell nuclear antigen (PCNA) in different histological and endoscopic diagnostic groups, in cases of Helicobacter pylori infection, in vivo. Paraffin embedded human gastric biopsy samples (86) were analysed by EGFR and PCNA immunohistochemistry and classified both on the basis of histology and endoscopic findings. In normal epithelia (NE), a positive correlation was found between PCNA and EGFR and in H. pylori-negative gastritis with and without intestinal metaplasia (P <0.01). On the other hand, a negative correlation was detected between the two immunohistochemical findings in H. pylori-associated gastritis with intestinal metaplasia (HPGIM) and in the atrophic gastritis (AG) group. In HPGIM the percentage of EGFR-positive cells was significantly lower (32.4 ± 30.4) when compared to either the NE (50.3 ± 23.7) or H. pylori-negative gastritis with intestinal metaplasia (HNGIM) (48.3 ± 23.7). In AG, EGFR was significantly lower when compared to the NE (P <0.05). Based on the endoscopic findings, a significant decrease of EGFR expression was found in gastric ulcer cases as compared to NE, gastritis or erosion cases (P <0.01). PCNA showed no significant alterations between the NE and gastritis, AG groups. The presence of H. pylori has an inverse effect on PCNA and EGFR expression in HPGIM.

AB - Our aim was to compare the expression of EGFR and proliferative cell nuclear antigen (PCNA) in different histological and endoscopic diagnostic groups, in cases of Helicobacter pylori infection, in vivo. Paraffin embedded human gastric biopsy samples (86) were analysed by EGFR and PCNA immunohistochemistry and classified both on the basis of histology and endoscopic findings. In normal epithelia (NE), a positive correlation was found between PCNA and EGFR and in H. pylori-negative gastritis with and without intestinal metaplasia (P <0.01). On the other hand, a negative correlation was detected between the two immunohistochemical findings in H. pylori-associated gastritis with intestinal metaplasia (HPGIM) and in the atrophic gastritis (AG) group. In HPGIM the percentage of EGFR-positive cells was significantly lower (32.4 ± 30.4) when compared to either the NE (50.3 ± 23.7) or H. pylori-negative gastritis with intestinal metaplasia (HNGIM) (48.3 ± 23.7). In AG, EGFR was significantly lower when compared to the NE (P <0.05). Based on the endoscopic findings, a significant decrease of EGFR expression was found in gastric ulcer cases as compared to NE, gastritis or erosion cases (P <0.01). PCNA showed no significant alterations between the NE and gastritis, AG groups. The presence of H. pylori has an inverse effect on PCNA and EGFR expression in HPGIM.

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