Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm

Wei Si, Ping Zheng, Yahui Li, A. Dinnyés, Weizhi Ji

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Glycerol and dimethyl sulfoxide (DMSO) are widely used as penetrating cryoprotectants in the freezing of sperm, and various concentrations are applied in different species and laboratories. The present study aimed to examine the effect of these two cryoprotectants at different concentrations (2%, 5%, 10%, and 15% glycerol or DMSO) on rhesus monkey sperm cryopreservation. The results showed that the highest recovery of post-thaw sperm motility, and plasma membrane and acrosome integrity was achieved when the sperm was frozen with 5% glycerol. Spermatozoa cryopreserved with 15% DMSO showed the lowest post-thaw sperm motility, and spermatozoa cryopreserved with 15% glycerol and 15% DMSO showed the lowest plasma membrane integrity among the eight groups. The results achieved with 5% glycerol were significantly better for all parameters than those obtained with 5% DMSO. The functional cryosurvival of sperm frozen with 5% glycerol was further assessed by in vitro fertilization (IVF). Overall, 85.7% of the oocytes were successfully fertilized, and 51.4% and 5.7% of the resulting zygotes developed into morulae and blastocysts, respectively. The results indicate that the type and concentration of the penetrating cryoprotectant used can greatly affect the survival of rhesus monkey sperm after it is frozen and thawed. The suitable glycerol level for rhesus monkey sperm freezing is 5%, and DMSO is not suitable for rhesus monkey sperm cryopreservation.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalAmerican Journal of Primatology
Volume62
Issue number4
DOIs
Publication statusPublished - Apr 2004

Fingerprint

cryopreservation
dimethyl sulfoxide
Macaca mulatta
sperm
glycerol
spermatozoa
cryoprotectants
motility
sperm motility
freezing
plasma membrane
membrane
effect
plasma
morula
in vitro fertilization
acrosome
zygote
blastocyst
oocytes

Keywords

  • Cryopreservation
  • DMSO
  • Glycerol
  • Rhesus monkey
  • Spermatozoa

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm. / Si, Wei; Zheng, Ping; Li, Yahui; Dinnyés, A.; Ji, Weizhi.

In: American Journal of Primatology, Vol. 62, No. 4, 04.2004, p. 301-306.

Research output: Contribution to journalArticle

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abstract = "Glycerol and dimethyl sulfoxide (DMSO) are widely used as penetrating cryoprotectants in the freezing of sperm, and various concentrations are applied in different species and laboratories. The present study aimed to examine the effect of these two cryoprotectants at different concentrations (2{\%}, 5{\%}, 10{\%}, and 15{\%} glycerol or DMSO) on rhesus monkey sperm cryopreservation. The results showed that the highest recovery of post-thaw sperm motility, and plasma membrane and acrosome integrity was achieved when the sperm was frozen with 5{\%} glycerol. Spermatozoa cryopreserved with 15{\%} DMSO showed the lowest post-thaw sperm motility, and spermatozoa cryopreserved with 15{\%} glycerol and 15{\%} DMSO showed the lowest plasma membrane integrity among the eight groups. The results achieved with 5{\%} glycerol were significantly better for all parameters than those obtained with 5{\%} DMSO. The functional cryosurvival of sperm frozen with 5{\%} glycerol was further assessed by in vitro fertilization (IVF). Overall, 85.7{\%} of the oocytes were successfully fertilized, and 51.4{\%} and 5.7{\%} of the resulting zygotes developed into morulae and blastocysts, respectively. The results indicate that the type and concentration of the penetrating cryoprotectant used can greatly affect the survival of rhesus monkey sperm after it is frozen and thawed. The suitable glycerol level for rhesus monkey sperm freezing is 5{\%}, and DMSO is not suitable for rhesus monkey sperm cryopreservation.",
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