Effect of genotype on the efficiency of mouse embryo cryopreservation by vitrification or slow freezing methods

A. Dinnyés, G. A. Wallace, W. F. Rall

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We examined possible genotype effects on the survival of 8‐ to 16‐cell mouse embryos isolated from four inbred strains (C57BL/6N, BALB/cAnN, DBA/2N, and C3H/HeN), a outbred stock (ICR), and various crosses after cryopreservation by vitrification or conventional slow freezing using glycerol solutions. The rates of in vitro development of C57BL/6N, BALB/cAnN, C3H/HeN, and ICR embryos to expanded blastocysts ranged from 86% to 94% after slow freezing and 85% to 97% after vitrification. The cryopreservation method did not significantly influence in vitro embryo survival after thawing (P >0.05). Although genotype significantly influenced the in vitro survival of embryos (P = 0.008), this presumably resulted from an increased difficulty in assessing the quality grade of C3H/HeN embryos prior to cryopreservation. The rates in vivo development of C57BL/6N, BALB/cAnN, C3H/HeN, DBA/2N, and ICR embryos to normal day 18–19 fetuses ranged from 19% to 64% after slow freezing and from 18% to 63% after vitrification. The in vivo development of cryopreserved embryos was significantly influenced by cryopreservation method and genotype (P = 0.01 and P = 0.001, respectively). Vitrification yielded significantly higher rates of in vivo development than that after slow freezing (P > 0.05). In vivo development rates of DBA/2N and ICR♀ X B6D2F1 ♂ embryos after cryopreservation were significantly higher than that of embryos from BALB/cAnN and C3H/HeN mice (P < 0.05). These results indicate that parental genotype exerts little or no effect on the ability of embryos to develop in vitro after vitrification or slow freezing. Differences in the ability of cryopreserved embryos to develop normally in vivo may reflect inherent genotype related differences in their post‐implantation developmental potential and not their sensitivity to cryoinjury. © 1995 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)429-435
Number of pages7
JournalMolecular Reproduction and Development
Volume40
Issue number4
DOIs
Publication statusPublished - Apr 1995

Keywords

  • Embryo banking
  • Genotype effects
  • Inbred strains
  • Vitrification

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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