Effect of genetic deletion and pharmacological antagonism of P2X7 receptors in a mouse animal model of migraine

Flóra Gölöncsér, B. Sperlágh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Purine receptors participate in peripheral and central sensitization and are associated with migraine headache. We investigated the role of P2X7 receptor (P2X7) in a nitroglycerin (NTG)-induced mouse model of migraine. Methods: Intraperitoneal NTG injection (15 mg/kg) triggered thermal hyperalgesia in the hindpaws of wild-type C57BL/6J mice, followed by the induction of c-fos in upper cervical spinal cord and trigeminal nucleus caudalis. The effect of genetic deletion of P2X7 and the selective P2X7 antagonist Brilliant Blue G (BBG) were examined on hyperalgesia and c-fos induction. Results: NTG decreased the paw withdrawal threshold in both wild-type and P2X7 knockout mice. Nevertheless, subacute BBG treatment (50 mg/kg/day i.p.) completely prevented the effect of NTG in wild-type, but not in knockout mice. Whereas P2X7 deficiency differentially affected the expression of c-fos, the average number of fos-immuno-reactive neurons in trigeminal nucleus caudalis, but not in upper cervical spinal cord was lower in BBG-treated wild-type mice after NTG treatment. Conclusions: Our results show that P2X7 receptors might participate in the pathogenesis of migraine, although upregulation of other P2X receptors probably compensate for the loss of its action in knockout mice. The data also suggest the therapeutic potential of P2X7 antagonists for the treatment of migraine.

Original languageEnglish
Article number24
Pages (from-to)1-8
Number of pages8
JournalThe journal of headache and pain
Volume15
Issue number1
DOIs
Publication statusPublished - Dec 1 2014

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Purinergic P2X7 Receptors
Nitroglycerin
Migraine Disorders
Animal Models
Pharmacology
Knockout Mice
Hyperalgesia
Spinal Trigeminal Nucleus
Central Nervous System Sensitization
Trigeminal Nuclei
Purinergic Receptors
Therapeutics
Intraperitoneal Injections
Inbred C57BL Mouse
Up-Regulation
Neurons
coomassie Brilliant Blue

Keywords

  • Brilliant blue G
  • migraine
  • mouse model
  • nitroglycerin
  • P2X7 receptor

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Clinical Neurology

Cite this

Effect of genetic deletion and pharmacological antagonism of P2X7 receptors in a mouse animal model of migraine. / Gölöncsér, Flóra; Sperlágh, B.

In: The journal of headache and pain, Vol. 15, No. 1, 24, 01.12.2014, p. 1-8.

Research output: Contribution to journalArticle

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