Effect of genetic deletion and pharmacological antagonism of P2X7 receptors in a mouse animal model of migraine

Flóra Gölöncsér, Beáta Sperlágh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Purine receptors participate in peripheral and central sensitization and are associated with migraine headache. We investigated the role of P2X7 receptor (P2X7) in a nitroglycerin (NTG)-induced mouse model of migraine. Methods: Intraperitoneal NTG injection (15 mg/kg) triggered thermal hyperalgesia in the hindpaws of wild-type C57BL/6J mice, followed by the induction of c-fos in upper cervical spinal cord and trigeminal nucleus caudalis. The effect of genetic deletion of P2X7 and the selective P2X7 antagonist Brilliant Blue G (BBG) were examined on hyperalgesia and c-fos induction. Results: NTG decreased the paw withdrawal threshold in both wild-type and P2X7 knockout mice. Nevertheless, subacute BBG treatment (50 mg/kg/day i.p.) completely prevented the effect of NTG in wild-type, but not in knockout mice. Whereas P2X7 deficiency differentially affected the expression of c-fos, the average number of fos-immuno-reactive neurons in trigeminal nucleus caudalis, but not in upper cervical spinal cord was lower in BBG-treated wild-type mice after NTG treatment. Conclusions: Our results show that P2X7 receptors might participate in the pathogenesis of migraine, although upregulation of other P2X receptors probably compensate for the loss of its action in knockout mice. The data also suggest the therapeutic potential of P2X7 antagonists for the treatment of migraine.

Original languageEnglish
Article number24
Pages (from-to)1-8
Number of pages8
JournalJournal of Headache and Pain
Volume15
Issue number1
DOIs
Publication statusPublished - Dec 1 2014

Keywords

  • Brilliant blue G
  • P2X7 receptor
  • migraine
  • mouse model
  • nitroglycerin

ASJC Scopus subject areas

  • Clinical Neurology
  • Anesthesiology and Pain Medicine

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