Effect of fructose 1-phosphate on the activation of liver glycogen synthase

P. Gergely, B. Toth, I. Farkas, G. Bot

Research output: Contribution to journalArticle

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Abstract

The activation (dephosphorylation) of glycogen synthase and the inactivation (dephosphorylation) of phosphorylase in rat liver extracts on the administration of fructose were examined. The lag in the conversion of synthase b into a was cancelled, owing to the accumulation of fructose 1-phosphate. A decrease in the rate of dephosphorylation of phosphorylase a was also observed. The latency re-appeared in gel-filtered liver extracts. Similar latency was demonstrated in extracts from glucagon-treated rats. Addition of fructose 1-phosphate to the extract was able to abolish the latency, and the activation of glycogen synthase and the inactivation of phosphorylase occurred simultaneously. Fructose 1-phosphate increased the activity of glycogen synthase b measured in the presence of 0.2-0.4 mM-glucose 6-phosphate. According to kinetic investigations, fructose 1-phosphate increased the affinity of synthase b for its substrate, UDP-glucose. The accumulation of fructose 1-phosphate resulted in glycogen synthesis in the liver by inducing the enzymic activity of glycogen synthase b in the presence of glucose 6-phosphate in vivo and by promoting the activation of glycogen synthase.

Original languageEnglish
Pages (from-to)133-137
Number of pages5
JournalBiochemical Journal
Volume232
Issue number1
Publication statusPublished - 1985

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Glycogen Synthase
Liver Glycogen
Chemical activation
Liver Extracts
Phosphorylases
Glucose-6-Phosphate
Rats
Phosphorylase a
Uridine Diphosphate Glucose
Fructose
Glucagon
Glycogen
Liver
Gels
fructose-1-phosphate
Kinetics
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Effect of fructose 1-phosphate on the activation of liver glycogen synthase. / Gergely, P.; Toth, B.; Farkas, I.; Bot, G.

In: Biochemical Journal, Vol. 232, No. 1, 1985, p. 133-137.

Research output: Contribution to journalArticle

Gergely, P, Toth, B, Farkas, I & Bot, G 1985, 'Effect of fructose 1-phosphate on the activation of liver glycogen synthase', Biochemical Journal, vol. 232, no. 1, pp. 133-137.
Gergely, P. ; Toth, B. ; Farkas, I. ; Bot, G. / Effect of fructose 1-phosphate on the activation of liver glycogen synthase. In: Biochemical Journal. 1985 ; Vol. 232, No. 1. pp. 133-137.
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