Effect of flavonoid on PGE2-induced alterations in percentage collagen synthesis in ossicle organ cultures

O. Ribari, I. Sziklai

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In adult guinea-pig stapes organ cultures, 3H (2, 3)-proline incorporation into the collagenase-digestible protein (CDP) and non-collagen protein (NCP) fractions of the ossicles was measured and the percentage of collagen synthesis (PCS) was calculated. Prostaglandin E2 (PGE2), a potent stimulator of bone resorption, inhibited the PCS in low concentrations (5 and 25 μM), whereas it stimulated it in pharmacological concentrations (50 and 100 μM). Ipriflavon, an isoflavone derivate of therapeutical potential in otosclerosis, also reduced the PCS in 1, 10 and 50 μM concentrations. 50 μM Ipriflavon stimulated the PCS inhibited by 5 μM PGE2, but decreased the PGE2-induced PCS enhancement in vitro.

Original languageEnglish
Pages (from-to)657-660
Number of pages4
JournalActa Oto-Laryngologica
Volume103
Issue number5-6
Publication statusPublished - 1987

Fingerprint

Organ Culture Techniques
Dinoprostone
Flavonoids
Collagen
Stapes
Otosclerosis
Isoflavones
Collagenases
Bone Resorption
Proline
Guinea Pigs
Proteins
Pharmacology

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Effect of flavonoid on PGE2-induced alterations in percentage collagen synthesis in ossicle organ cultures. / Ribari, O.; Sziklai, I.

In: Acta Oto-Laryngologica, Vol. 103, No. 5-6, 1987, p. 657-660.

Research output: Contribution to journalArticle

@article{0bb6fa91e0d347dd9500cf706aa9ebca,
title = "Effect of flavonoid on PGE2-induced alterations in percentage collagen synthesis in ossicle organ cultures",
abstract = "In adult guinea-pig stapes organ cultures, 3H (2, 3)-proline incorporation into the collagenase-digestible protein (CDP) and non-collagen protein (NCP) fractions of the ossicles was measured and the percentage of collagen synthesis (PCS) was calculated. Prostaglandin E2 (PGE2), a potent stimulator of bone resorption, inhibited the PCS in low concentrations (5 and 25 μM), whereas it stimulated it in pharmacological concentrations (50 and 100 μM). Ipriflavon, an isoflavone derivate of therapeutical potential in otosclerosis, also reduced the PCS in 1, 10 and 50 μM concentrations. 50 μM Ipriflavon stimulated the PCS inhibited by 5 μM PGE2, but decreased the PGE2-induced PCS enhancement in vitro.",
author = "O. Ribari and I. Sziklai",
year = "1987",
language = "English",
volume = "103",
pages = "657--660",
journal = "Acta Oto-Laryngologica",
issn = "0001-6489",
publisher = "Informa Healthcare",
number = "5-6",

}

TY - JOUR

T1 - Effect of flavonoid on PGE2-induced alterations in percentage collagen synthesis in ossicle organ cultures

AU - Ribari, O.

AU - Sziklai, I.

PY - 1987

Y1 - 1987

N2 - In adult guinea-pig stapes organ cultures, 3H (2, 3)-proline incorporation into the collagenase-digestible protein (CDP) and non-collagen protein (NCP) fractions of the ossicles was measured and the percentage of collagen synthesis (PCS) was calculated. Prostaglandin E2 (PGE2), a potent stimulator of bone resorption, inhibited the PCS in low concentrations (5 and 25 μM), whereas it stimulated it in pharmacological concentrations (50 and 100 μM). Ipriflavon, an isoflavone derivate of therapeutical potential in otosclerosis, also reduced the PCS in 1, 10 and 50 μM concentrations. 50 μM Ipriflavon stimulated the PCS inhibited by 5 μM PGE2, but decreased the PGE2-induced PCS enhancement in vitro.

AB - In adult guinea-pig stapes organ cultures, 3H (2, 3)-proline incorporation into the collagenase-digestible protein (CDP) and non-collagen protein (NCP) fractions of the ossicles was measured and the percentage of collagen synthesis (PCS) was calculated. Prostaglandin E2 (PGE2), a potent stimulator of bone resorption, inhibited the PCS in low concentrations (5 and 25 μM), whereas it stimulated it in pharmacological concentrations (50 and 100 μM). Ipriflavon, an isoflavone derivate of therapeutical potential in otosclerosis, also reduced the PCS in 1, 10 and 50 μM concentrations. 50 μM Ipriflavon stimulated the PCS inhibited by 5 μM PGE2, but decreased the PGE2-induced PCS enhancement in vitro.

UR - http://www.scopus.com/inward/record.url?scp=0023161618&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023161618&partnerID=8YFLogxK

M3 - Article

C2 - 3475954

AN - SCOPUS:0023161618

VL - 103

SP - 657

EP - 660

JO - Acta Oto-Laryngologica

JF - Acta Oto-Laryngologica

SN - 0001-6489

IS - 5-6

ER -