Effect of feeding Bacillus cereus var. toyoi and/or mannan oligosaccharide (MOS) on blood clinical chemistry, oxidative stress, immune response and genotoxicity in T-2 toxin exposed rabbits

Dóra Hafner, T. Tuboly, M. Mézes, Zsófia Bloch-Bodnár, K. Balogh, Viola Vántus, Brigitta Bóta, Judit Szabó-Fodor, Zsolt Matics, András Szabó, Melinda Kovács

Research output: Contribution to journalArticle

Abstract

The aim of the study was to determine the effect of Bacillus cereus var. toyoi (200 mg/kg) and mannan oligosaccharide (MOS, 2 g/kg) in T-2 toxin exposed rabbits. Weaned rabbits (35 days of age) were assigned into four experimental groups (n = 20 in each): control (C), B. cereus (B), MOS (M) and B. cereus+MOS (BM) group. At 49 days of age, rabbits were divided into two groups (n = 10 in each), and half of the C, B, M and BM rabbits were fed with the same diet as before, but contaminated with 2 mg/kg feed T-2 toxin (groups CT, BT, MT and BMT). On day 50 and 60, rabbits were injected with ovalbumin. At 70 days of age, all animals were sacrificed. Daily weight gain was smaller in toxin challenged animals (17.6–19.5 g) compared to controls (29.7 g). Serum total protein concentration was less in toxin treated animals, among them in CT group (49.7 g/L) it decreased underneath the reference range. T-2 increased the glutathione peroxidase activity in the kidney. C and B treatments resulted in the highest IgG production. Phagocytic activity was the lowest in CT animals (76.4%), while the highest in C and BM groups (82%). Cytokine expression in the intestinal mucosa was lower in T-2 exposed animals as compared to their relevant controls. None of the feed additives had any effect on the T-2 induced changes in the oxidative stress and immune response. T-2 had slight genotoxic effect on lymphocytes, which was alleviated by MOS and B. cereus.Highlights The suspected protective effect of Bacillus cereus var. toyoi and mannan oligosaccharide was investigated in T-2 toxin exposed rabbits. T-2 impaired immune response and was slightly genotoxic on lymphocytes. MOS and B. cereus alleviated the genotoxicity of T-2.

Original languageEnglish
Pages (from-to)1239-1251
Number of pages13
JournalItalian Journal of Animal Science
Volume18
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

Bacillus cereus biovar toyoi
T-2 toxin
genotoxicity
oligosaccharides
Bacillus cereus
chemistry
oxidative stress
rabbits
immune response
blood
animals
toxins
lymphocytes
feed additives
ovalbumin
intestinal mucosa
phagocytosis
glutathione peroxidase
blood proteins
protective effect

Keywords

  • Bacillus cereus var. toyoi
  • mannan oligosaccharide
  • rabbit
  • T-2 toxin

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Effect of feeding Bacillus cereus var. toyoi and/or mannan oligosaccharide (MOS) on blood clinical chemistry, oxidative stress, immune response and genotoxicity in T-2 toxin exposed rabbits. / Hafner, Dóra; Tuboly, T.; Mézes, M.; Bloch-Bodnár, Zsófia; Balogh, K.; Vántus, Viola; Bóta, Brigitta; Szabó-Fodor, Judit; Matics, Zsolt; Szabó, András; Kovács, Melinda.

In: Italian Journal of Animal Science, Vol. 18, No. 1, 01.01.2019, p. 1239-1251.

Research output: Contribution to journalArticle

Hafner, Dóra ; Tuboly, T. ; Mézes, M. ; Bloch-Bodnár, Zsófia ; Balogh, K. ; Vántus, Viola ; Bóta, Brigitta ; Szabó-Fodor, Judit ; Matics, Zsolt ; Szabó, András ; Kovács, Melinda. / Effect of feeding Bacillus cereus var. toyoi and/or mannan oligosaccharide (MOS) on blood clinical chemistry, oxidative stress, immune response and genotoxicity in T-2 toxin exposed rabbits. In: Italian Journal of Animal Science. 2019 ; Vol. 18, No. 1. pp. 1239-1251.
@article{0b771107c1894e4e9833be092df27159,
title = "Effect of feeding Bacillus cereus var. toyoi and/or mannan oligosaccharide (MOS) on blood clinical chemistry, oxidative stress, immune response and genotoxicity in T-2 toxin exposed rabbits",
abstract = "The aim of the study was to determine the effect of Bacillus cereus var. toyoi (200 mg/kg) and mannan oligosaccharide (MOS, 2 g/kg) in T-2 toxin exposed rabbits. Weaned rabbits (35 days of age) were assigned into four experimental groups (n = 20 in each): control (C), B. cereus (B), MOS (M) and B. cereus+MOS (BM) group. At 49 days of age, rabbits were divided into two groups (n = 10 in each), and half of the C, B, M and BM rabbits were fed with the same diet as before, but contaminated with 2 mg/kg feed T-2 toxin (groups CT, BT, MT and BMT). On day 50 and 60, rabbits were injected with ovalbumin. At 70 days of age, all animals were sacrificed. Daily weight gain was smaller in toxin challenged animals (17.6–19.5 g) compared to controls (29.7 g). Serum total protein concentration was less in toxin treated animals, among them in CT group (49.7 g/L) it decreased underneath the reference range. T-2 increased the glutathione peroxidase activity in the kidney. C and B treatments resulted in the highest IgG production. Phagocytic activity was the lowest in CT animals (76.4{\%}), while the highest in C and BM groups (82{\%}). Cytokine expression in the intestinal mucosa was lower in T-2 exposed animals as compared to their relevant controls. None of the feed additives had any effect on the T-2 induced changes in the oxidative stress and immune response. T-2 had slight genotoxic effect on lymphocytes, which was alleviated by MOS and B. cereus.Highlights The suspected protective effect of Bacillus cereus var. toyoi and mannan oligosaccharide was investigated in T-2 toxin exposed rabbits. T-2 impaired immune response and was slightly genotoxic on lymphocytes. MOS and B. cereus alleviated the genotoxicity of T-2.",
keywords = "Bacillus cereus var. toyoi, mannan oligosaccharide, rabbit, T-2 toxin",
author = "D{\'o}ra Hafner and T. Tuboly and M. M{\'e}zes and Zs{\'o}fia Bloch-Bodn{\'a}r and K. Balogh and Viola V{\'a}ntus and Brigitta B{\'o}ta and Judit Szab{\'o}-Fodor and Zsolt Matics and Andr{\'a}s Szab{\'o} and Melinda Kov{\'a}cs",
year = "2019",
month = "1",
day = "1",
doi = "10.1080/1828051X.2019.1641165",
language = "English",
volume = "18",
pages = "1239--1251",
journal = "Italian Journal of Animal Science",
issn = "1594-4077",
publisher = "PagePress",
number = "1",

}

TY - JOUR

T1 - Effect of feeding Bacillus cereus var. toyoi and/or mannan oligosaccharide (MOS) on blood clinical chemistry, oxidative stress, immune response and genotoxicity in T-2 toxin exposed rabbits

AU - Hafner, Dóra

AU - Tuboly, T.

AU - Mézes, M.

AU - Bloch-Bodnár, Zsófia

AU - Balogh, K.

AU - Vántus, Viola

AU - Bóta, Brigitta

AU - Szabó-Fodor, Judit

AU - Matics, Zsolt

AU - Szabó, András

AU - Kovács, Melinda

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The aim of the study was to determine the effect of Bacillus cereus var. toyoi (200 mg/kg) and mannan oligosaccharide (MOS, 2 g/kg) in T-2 toxin exposed rabbits. Weaned rabbits (35 days of age) were assigned into four experimental groups (n = 20 in each): control (C), B. cereus (B), MOS (M) and B. cereus+MOS (BM) group. At 49 days of age, rabbits were divided into two groups (n = 10 in each), and half of the C, B, M and BM rabbits were fed with the same diet as before, but contaminated with 2 mg/kg feed T-2 toxin (groups CT, BT, MT and BMT). On day 50 and 60, rabbits were injected with ovalbumin. At 70 days of age, all animals were sacrificed. Daily weight gain was smaller in toxin challenged animals (17.6–19.5 g) compared to controls (29.7 g). Serum total protein concentration was less in toxin treated animals, among them in CT group (49.7 g/L) it decreased underneath the reference range. T-2 increased the glutathione peroxidase activity in the kidney. C and B treatments resulted in the highest IgG production. Phagocytic activity was the lowest in CT animals (76.4%), while the highest in C and BM groups (82%). Cytokine expression in the intestinal mucosa was lower in T-2 exposed animals as compared to their relevant controls. None of the feed additives had any effect on the T-2 induced changes in the oxidative stress and immune response. T-2 had slight genotoxic effect on lymphocytes, which was alleviated by MOS and B. cereus.Highlights The suspected protective effect of Bacillus cereus var. toyoi and mannan oligosaccharide was investigated in T-2 toxin exposed rabbits. T-2 impaired immune response and was slightly genotoxic on lymphocytes. MOS and B. cereus alleviated the genotoxicity of T-2.

AB - The aim of the study was to determine the effect of Bacillus cereus var. toyoi (200 mg/kg) and mannan oligosaccharide (MOS, 2 g/kg) in T-2 toxin exposed rabbits. Weaned rabbits (35 days of age) were assigned into four experimental groups (n = 20 in each): control (C), B. cereus (B), MOS (M) and B. cereus+MOS (BM) group. At 49 days of age, rabbits were divided into two groups (n = 10 in each), and half of the C, B, M and BM rabbits were fed with the same diet as before, but contaminated with 2 mg/kg feed T-2 toxin (groups CT, BT, MT and BMT). On day 50 and 60, rabbits were injected with ovalbumin. At 70 days of age, all animals were sacrificed. Daily weight gain was smaller in toxin challenged animals (17.6–19.5 g) compared to controls (29.7 g). Serum total protein concentration was less in toxin treated animals, among them in CT group (49.7 g/L) it decreased underneath the reference range. T-2 increased the glutathione peroxidase activity in the kidney. C and B treatments resulted in the highest IgG production. Phagocytic activity was the lowest in CT animals (76.4%), while the highest in C and BM groups (82%). Cytokine expression in the intestinal mucosa was lower in T-2 exposed animals as compared to their relevant controls. None of the feed additives had any effect on the T-2 induced changes in the oxidative stress and immune response. T-2 had slight genotoxic effect on lymphocytes, which was alleviated by MOS and B. cereus.Highlights The suspected protective effect of Bacillus cereus var. toyoi and mannan oligosaccharide was investigated in T-2 toxin exposed rabbits. T-2 impaired immune response and was slightly genotoxic on lymphocytes. MOS and B. cereus alleviated the genotoxicity of T-2.

KW - Bacillus cereus var. toyoi

KW - mannan oligosaccharide

KW - rabbit

KW - T-2 toxin

UR - http://www.scopus.com/inward/record.url?scp=85070896607&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85070896607&partnerID=8YFLogxK

U2 - 10.1080/1828051X.2019.1641165

DO - 10.1080/1828051X.2019.1641165

M3 - Article

VL - 18

SP - 1239

EP - 1251

JO - Italian Journal of Animal Science

JF - Italian Journal of Animal Science

SN - 1594-4077

IS - 1

ER -