Effect of extenders on sperm cryopreservation of African catfish, Clarias gariepinus (Burchell)

B. Urbányi, A. Horváth, Z. Varga, L. Horváth, I. Magyary, F. Radics

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The effect of extenders was studied on the cryopreservation of sperm from African catfish, Clarias gariepinus (Burchell). The following six basic extenders were tested: fructose, glucose, sucrose, NaCl, KCl solutions and the artificial seminal plasma of the African catfish. Each of these extenders was tested both with and without buffer systems (i.e. NaHCO3-CO2 and Tris-HCl) by using 10% dimethylsulphoxide (DMSO) as a cryoprotectant. The two-step freezing was carried out in a programmable freezer by using the following freezing rates: (1) 4 °C min-1 between 3 and -4 °C; (2) and 11 °C min-1 between -4 and -80 °C. The best post-thaw motility (25%) was achieved with 333 mmol L-1 fructose solution and NaHCO3 buffer. The fertilization experiments were carried out with unbuffered fructose and glucose extenders using various amounts of sperm and two fertilization methods: (1) dry and (2) wet. The best fertilization rates were achieved with 75 μL of sperm and wet fertilization with glucose extender, or 100 μL of sperm and dry fertilization in case of fructose - both methods fertilized 96% of all eggs.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalAquaculture Research
Volume30
Issue number2
DOIs
Publication statusPublished - Feb 1999

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cryopreservation
Clarias gariepinus
catfish
sperm
fructose
spermatozoa
glucose
freezing
buffers
freezers
seminal plasma
motility
cryoprotectants
dimethyl sulfoxide
sucrose
egg
plasma
effect
methodology
experiment

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Effect of extenders on sperm cryopreservation of African catfish, Clarias gariepinus (Burchell). / Urbányi, B.; Horváth, A.; Varga, Z.; Horváth, L.; Magyary, I.; Radics, F.

In: Aquaculture Research, Vol. 30, No. 2, 02.1999, p. 145-151.

Research output: Contribution to journalArticle

Urbányi, B. ; Horváth, A. ; Varga, Z. ; Horváth, L. ; Magyary, I. ; Radics, F. / Effect of extenders on sperm cryopreservation of African catfish, Clarias gariepinus (Burchell). In: Aquaculture Research. 1999 ; Vol. 30, No. 2. pp. 145-151.
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