Effect of exposure to ceramic fibres and cigarette smoke on antioxidant status of the lung

Zuzana Kováčiková, Marta Hurnánková, Silvia Černá, E. Tátrai, Milan Beňo, Soňa Wimmerová

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives: The programmes of asbestos replacement brought the need to use other fibres for insulation or reinforcement of material. The aim of the presented study was to follow the effect of refractory ceramic fibres (RCF3) alone or in combination with cigarette smoke (CS) on antioxidant status of the lung in experiment on animals. As free radicals are supposed to play a role in pathogenesis of lung diseases and the toxicity of particles has been associated with production of reactive oxygen species, the antioxidant status may serve as marker of lung injury. Our hypothesis was that the effect of combined exposure to RCF3 and CS will be additive or synergic. Design: Scheme of experiment: Wistar rats were randomly divided into 4 groups per 6 animals: control, intratracheal exposure to 4 mg of RCF3, inhalatory exposure to mainstream of cigarette smoke from 8 standard research 1R1 cigarettes per day, and both intratracheal exposure to RCF3 and inhalatory to CS. The exposure lasted 6 months, the inhalatory exposure was performed 5 times per week. After finishing the exposure bronchoalveolar lavage of lungs was performed and ascorbic acid, superoxide dismutase, glutathione and glutathione peroxidase were determined in lung tissue and cell free fraction of bronchoalveolar lavage fluid (BALF). Results: The results showed that the most sensitive indicator of changes in anti-oxidant status was glutathione, which was changed in all groups both in BALF and lung tissue homogenate.

Original languageEnglish
Pages (from-to)23-26
Number of pages4
JournalNeuroendocrinology Letters
Volume27
Issue numberSUPPL. 2
Publication statusPublished - Dec 2006

Fingerprint

Ceramics
Smoke
Tobacco Products
Antioxidants
Lung
Bronchoalveolar Lavage Fluid
Glutathione
Asbestos
Lung Injury
Bronchoalveolar Lavage
Glutathione Peroxidase
Oxidants
Lung Diseases
Ascorbic Acid
Superoxide Dismutase
Free Radicals
Wistar Rats
Reactive Oxygen Species
Research

Keywords

  • Antioxidant status
  • Cigarette smoke
  • Combined exposure
  • Lung
  • Refractory ceramic fibres

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Kováčiková, Z., Hurnánková, M., Černá, S., Tátrai, E., Beňo, M., & Wimmerová, S. (2006). Effect of exposure to ceramic fibres and cigarette smoke on antioxidant status of the lung. Neuroendocrinology Letters, 27(SUPPL. 2), 23-26.

Effect of exposure to ceramic fibres and cigarette smoke on antioxidant status of the lung. / Kováčiková, Zuzana; Hurnánková, Marta; Černá, Silvia; Tátrai, E.; Beňo, Milan; Wimmerová, Soňa.

In: Neuroendocrinology Letters, Vol. 27, No. SUPPL. 2, 12.2006, p. 23-26.

Research output: Contribution to journalArticle

Kováčiková, Z, Hurnánková, M, Černá, S, Tátrai, E, Beňo, M & Wimmerová, S 2006, 'Effect of exposure to ceramic fibres and cigarette smoke on antioxidant status of the lung', Neuroendocrinology Letters, vol. 27, no. SUPPL. 2, pp. 23-26.
Kováčiková Z, Hurnánková M, Černá S, Tátrai E, Beňo M, Wimmerová S. Effect of exposure to ceramic fibres and cigarette smoke on antioxidant status of the lung. Neuroendocrinology Letters. 2006 Dec;27(SUPPL. 2):23-26.
Kováčiková, Zuzana ; Hurnánková, Marta ; Černá, Silvia ; Tátrai, E. ; Beňo, Milan ; Wimmerová, Soňa. / Effect of exposure to ceramic fibres and cigarette smoke on antioxidant status of the lung. In: Neuroendocrinology Letters. 2006 ; Vol. 27, No. SUPPL. 2. pp. 23-26.
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