Effect of exogenous circulating anti-bPL antibodies on bovine placental lactogen measurements in foetal samples

Andrea V. Alvarez-Oxiley, Noelita M. de Sousa, Jean Luc Hornick, Kamal Touati, Gysbert C. van der Weijden, Marcel A M Taverne, O. Szenci, Jean François Beckers

Research output: Contribution to journalArticle

Abstract

Background: The involvement of placental lactogen (PL) in the regulation of foetal growth has been investigated in different species by in vivo immunomodulation techniques. However, when circulating antibodies are present together with the hormone, the procedure for hormonal measurement becomes considerably complex. The aim of this study was the immunoneutralization of bovine placental lactogen (bPL) concentrations in bovine foetal circulation by direct infusion of rabbit anti-bPL purified immunoglobulins (IgG) via a foetal catheter (in vivo study). The ability of a RIA based on guinea pig anti-bPL antiserum, for the measurement of bPL concentrations in samples containing exogenous rabbit anti-bPL immunoglobulins, was also analyzed in in vitro and in vivo conditions.Methods: Six bovine foetuses were chronic cannulated on the aorta via the medial tarsal artery. Infusion of rabbit anti-bPL IgG was performed during late gestation. Pooled rabbit anti-bPL antisera had a maximal neutralization capacity of 25 μg bPL/mL of immunoglobulin. Interference of rabbit anti-bPL immunoglobulin with radioimmunoassay measurement using guinea pig anti-bPL as primary antibody was first evaluated in vitro. Polyclonal anti-bPL antibodies raised in rabbit were added in foetal sera to produce 100 samples with known antibodies titers (dilutions ranging from 1:2,500 till 1:1,280,000).Result(s): Assessment of the interference of rabbit anti-bPL antibody showed that bPL concentrations were significantly lower (P <0.05) in samples added with dilutions of rabbit antiserum lower than 1:80,000 (one foetus) or 1:10,000 (four foetuses). It was also shown that the recovery of added bPL (12 ng/mL) was markedly reduced in those samples in which exogenous rabbit anti-bPL were added at dilutions lower than 1:20,000. Concentrations of foetal bPL were determined in samples from cannulated foetuses. In foetuses 1 and 6, bPL concentrations remained almost unchanged (

Original languageEnglish
Article number9
JournalActa Veterinaria Scandinavica
Volume52
Issue number1
DOIs
Publication statusPublished - Feb 3 2010

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choriomammotropin
Placental Lactogen
antibodies
Antibodies
cattle
rabbits
Rabbits
sampling
fetus
Fetus
immunoglobulins
Immunoglobulins
antiserum
Immune Sera
guinea pigs
Guinea Pigs
Immunoglobulin G

ASJC Scopus subject areas

  • veterinary(all)

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Alvarez-Oxiley, A. V., de Sousa, N. M., Hornick, J. L., Touati, K., van der Weijden, G. C., Taverne, M. A. M., ... Beckers, J. F. (2010). Effect of exogenous circulating anti-bPL antibodies on bovine placental lactogen measurements in foetal samples. Acta Veterinaria Scandinavica, 52(1), [9]. https://doi.org/10.1186/1751-0147-52-9

Effect of exogenous circulating anti-bPL antibodies on bovine placental lactogen measurements in foetal samples. / Alvarez-Oxiley, Andrea V.; de Sousa, Noelita M.; Hornick, Jean Luc; Touati, Kamal; van der Weijden, Gysbert C.; Taverne, Marcel A M; Szenci, O.; Beckers, Jean François.

In: Acta Veterinaria Scandinavica, Vol. 52, No. 1, 9, 03.02.2010.

Research output: Contribution to journalArticle

Alvarez-Oxiley, AV, de Sousa, NM, Hornick, JL, Touati, K, van der Weijden, GC, Taverne, MAM, Szenci, O & Beckers, JF 2010, 'Effect of exogenous circulating anti-bPL antibodies on bovine placental lactogen measurements in foetal samples', Acta Veterinaria Scandinavica, vol. 52, no. 1, 9. https://doi.org/10.1186/1751-0147-52-9
Alvarez-Oxiley, Andrea V. ; de Sousa, Noelita M. ; Hornick, Jean Luc ; Touati, Kamal ; van der Weijden, Gysbert C. ; Taverne, Marcel A M ; Szenci, O. ; Beckers, Jean François. / Effect of exogenous circulating anti-bPL antibodies on bovine placental lactogen measurements in foetal samples. In: Acta Veterinaria Scandinavica. 2010 ; Vol. 52, No. 1.
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abstract = "Background: The involvement of placental lactogen (PL) in the regulation of foetal growth has been investigated in different species by in vivo immunomodulation techniques. However, when circulating antibodies are present together with the hormone, the procedure for hormonal measurement becomes considerably complex. The aim of this study was the immunoneutralization of bovine placental lactogen (bPL) concentrations in bovine foetal circulation by direct infusion of rabbit anti-bPL purified immunoglobulins (IgG) via a foetal catheter (in vivo study). The ability of a RIA based on guinea pig anti-bPL antiserum, for the measurement of bPL concentrations in samples containing exogenous rabbit anti-bPL immunoglobulins, was also analyzed in in vitro and in vivo conditions.Methods: Six bovine foetuses were chronic cannulated on the aorta via the medial tarsal artery. Infusion of rabbit anti-bPL IgG was performed during late gestation. Pooled rabbit anti-bPL antisera had a maximal neutralization capacity of 25 μg bPL/mL of immunoglobulin. Interference of rabbit anti-bPL immunoglobulin with radioimmunoassay measurement using guinea pig anti-bPL as primary antibody was first evaluated in vitro. Polyclonal anti-bPL antibodies raised in rabbit were added in foetal sera to produce 100 samples with known antibodies titers (dilutions ranging from 1:2,500 till 1:1,280,000).Result(s): Assessment of the interference of rabbit anti-bPL antibody showed that bPL concentrations were significantly lower (P <0.05) in samples added with dilutions of rabbit antiserum lower than 1:80,000 (one foetus) or 1:10,000 (four foetuses). It was also shown that the recovery of added bPL (12 ng/mL) was markedly reduced in those samples in which exogenous rabbit anti-bPL were added at dilutions lower than 1:20,000. Concentrations of foetal bPL were determined in samples from cannulated foetuses. In foetuses 1 and 6, bPL concentrations remained almost unchanged (",
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AU - Touati, Kamal

AU - van der Weijden, Gysbert C.

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