Effect of elastin peptides and N-formyl-methionyl-leucyl phenylalanine on cytosolic free calcium in polymorphonuclear leukocytes of healthy middle-aged and elderly subjects

Z. Varga, Éva M. Kovács, G. Paragh, Marie Paule Jacob, Ladislas Robert, Tamás Fülöp

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The effect of elastin peptides (kappa-elastin, KE) and N-formyl-methionyl-leucyl-phenylalanine (FMLP) on cytosolic free calcium in polymorphonuclear leukocytes (PMNLs) of healthy middle-aged (35-45 years) and elderly (>60 years) patients with normal and high serum cholesterol level was investigated. The cytosolic free calcium ([Ca2+]iti) elevation after stimulation with these compounds was decreased in PMNLs of the aged groups compared to the healthy middle-aged group. The guanine nucleotide binding regulatory Giti protein inhibitor, pertussis toxin (PT) prevented the enhancing effect of KE and FMLP on PMNL free calcium of healthy middle-aged subjects, but could not completely abolish the [Ca2+]iti elevation in PMNLs of aged subjects.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalClinical Biochemistry
Volume21
Issue number2
DOIs
Publication statusPublished - 1988

Fingerprint

N-Formylmethionine Leucyl-Phenylalanine
Elastin
Neutrophils
Calcium
Peptides
methionyl-leucyl-phenylalanine
Guanine Nucleotides
Pertussis Toxin
Hypercholesterolemia
Cholesterol
Proteins
Serum

Keywords

  • aging
  • chemotactic peptide
  • cytosolic free calcium
  • elastin peptides
  • polymorphonuclear leukocytes

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Effect of elastin peptides and N-formyl-methionyl-leucyl phenylalanine on cytosolic free calcium in polymorphonuclear leukocytes of healthy middle-aged and elderly subjects. / Varga, Z.; Kovács, Éva M.; Paragh, G.; Jacob, Marie Paule; Robert, Ladislas; Fülöp, Tamás.

In: Clinical Biochemistry, Vol. 21, No. 2, 1988, p. 127-130.

Research output: Contribution to journalArticle

@article{695db08e4e5c41e0bf3ccefc2d5d10cf,
title = "Effect of elastin peptides and N-formyl-methionyl-leucyl phenylalanine on cytosolic free calcium in polymorphonuclear leukocytes of healthy middle-aged and elderly subjects",
abstract = "The effect of elastin peptides (kappa-elastin, KE) and N-formyl-methionyl-leucyl-phenylalanine (FMLP) on cytosolic free calcium in polymorphonuclear leukocytes (PMNLs) of healthy middle-aged (35-45 years) and elderly (>60 years) patients with normal and high serum cholesterol level was investigated. The cytosolic free calcium ([Ca2+]iti) elevation after stimulation with these compounds was decreased in PMNLs of the aged groups compared to the healthy middle-aged group. The guanine nucleotide binding regulatory Giti protein inhibitor, pertussis toxin (PT) prevented the enhancing effect of KE and FMLP on PMNL free calcium of healthy middle-aged subjects, but could not completely abolish the [Ca2+]iti elevation in PMNLs of aged subjects.",
keywords = "aging, chemotactic peptide, cytosolic free calcium, elastin peptides, polymorphonuclear leukocytes",
author = "Z. Varga and Kov{\'a}cs, {{\'E}va M.} and G. Paragh and Jacob, {Marie Paule} and Ladislas Robert and Tam{\'a}s F{\"u}l{\"o}p",
year = "1988",
doi = "10.1016/S0009-9120(88)80101-2",
language = "English",
volume = "21",
pages = "127--130",
journal = "Clinical Biochemistry",
issn = "0009-9120",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Effect of elastin peptides and N-formyl-methionyl-leucyl phenylalanine on cytosolic free calcium in polymorphonuclear leukocytes of healthy middle-aged and elderly subjects

AU - Varga, Z.

AU - Kovács, Éva M.

AU - Paragh, G.

AU - Jacob, Marie Paule

AU - Robert, Ladislas

AU - Fülöp, Tamás

PY - 1988

Y1 - 1988

N2 - The effect of elastin peptides (kappa-elastin, KE) and N-formyl-methionyl-leucyl-phenylalanine (FMLP) on cytosolic free calcium in polymorphonuclear leukocytes (PMNLs) of healthy middle-aged (35-45 years) and elderly (>60 years) patients with normal and high serum cholesterol level was investigated. The cytosolic free calcium ([Ca2+]iti) elevation after stimulation with these compounds was decreased in PMNLs of the aged groups compared to the healthy middle-aged group. The guanine nucleotide binding regulatory Giti protein inhibitor, pertussis toxin (PT) prevented the enhancing effect of KE and FMLP on PMNL free calcium of healthy middle-aged subjects, but could not completely abolish the [Ca2+]iti elevation in PMNLs of aged subjects.

AB - The effect of elastin peptides (kappa-elastin, KE) and N-formyl-methionyl-leucyl-phenylalanine (FMLP) on cytosolic free calcium in polymorphonuclear leukocytes (PMNLs) of healthy middle-aged (35-45 years) and elderly (>60 years) patients with normal and high serum cholesterol level was investigated. The cytosolic free calcium ([Ca2+]iti) elevation after stimulation with these compounds was decreased in PMNLs of the aged groups compared to the healthy middle-aged group. The guanine nucleotide binding regulatory Giti protein inhibitor, pertussis toxin (PT) prevented the enhancing effect of KE and FMLP on PMNL free calcium of healthy middle-aged subjects, but could not completely abolish the [Ca2+]iti elevation in PMNLs of aged subjects.

KW - aging

KW - chemotactic peptide

KW - cytosolic free calcium

KW - elastin peptides

KW - polymorphonuclear leukocytes

UR - http://www.scopus.com/inward/record.url?scp=0023920088&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023920088&partnerID=8YFLogxK

U2 - 10.1016/S0009-9120(88)80101-2

DO - 10.1016/S0009-9120(88)80101-2

M3 - Article

VL - 21

SP - 127

EP - 130

JO - Clinical Biochemistry

JF - Clinical Biochemistry

SN - 0009-9120

IS - 2

ER -