Effect of EGF on rat parotid gland secretory function

Karnam R. Purushotham, Tivadar Zelles, Josef Blazsek, Paoli Wang, George A. Paul, Micah Kerr, Michael G. Humphreys-Beher

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8 Citations (Scopus)

Abstract

The present study reports changes in saliva composition from the rat parotid gland in response to single and repeated administration of epidermal growth factor (EGF). Treatment of rats with EGF (10 μg/kg, i.p., twice daily for 3 days) caused an increase in amylase activity in saliva collected from cannulated parotid duct, following stimulation of secretion with pilocarpine, with a corresponding decrease in enzyme activity in the gland. Analysis of parotid gland RNA by reverse transcriptase-PCR generated a single predicted amylase-derived cDNA product of 576 bp. The steady-state levels of mRNA for amylase from EGF-treated parotid total RNA showed a 1.8-fold increase compared to untreated controls. A single dose of EGF (15 min following i.p. injection) elicited an activation of both protein kinase A and protein kinase C activities. While the activation of protein kinase A was still maintained under the chronic EGF regimen, the activity levels of protein kinase C showed down-regulation to untreated control values.

Original languageEnglish
Pages (from-to)7-14
Number of pages8
JournalComparative Biochemistry and Physiology. Part C: Comparative
Volume110
Issue number1
DOIs
Publication statusPublished - Jan 1995

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Keywords

  • Amylase
  • Growth factors
  • Salivary glands
  • Secretion
  • Signal transduction

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Purushotham, K. R., Zelles, T., Blazsek, J., Wang, P., Paul, G. A., Kerr, M., & Humphreys-Beher, M. G. (1995). Effect of EGF on rat parotid gland secretory function. Comparative Biochemistry and Physiology. Part C: Comparative, 110(1), 7-14. https://doi.org/10.1016/0742-8413(94)00083-M