Biogázüzemi fermentlé és Phylazonit MC baktériumtrágya hatása a silókukorica zöldtömegére és a talaj biológiai aktivitására

Translated title of the contribution: Effect of digestate and Phylazonit MC on the yield of silage maize and the biological activity of the soil

Marianna Makádi, Attila Tomócsik, Viktória Orosz, József Lengyel, Borbála Biró, Árpád Márton

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Abstract

The effect of digestate originating from a biogas plant and of Phylazonit MC bacterium fertilizer on the fresh mass yield of silage maize (Zea mays L. 'Coralba') grown as a secondary crop and on the biological activity of the soil was studied in a pilot experiment in Nyírbátor, E. Hungary, set up in July 2006 on plots measuring 100x30 m, previously sown to wheat. Soil and plant samples were taken in three replications from randomly selected representative points of inner plots. The soil was of sandy texture with pH(KCl): 4.33, y1: 16.5, KA: 29 and humus: 0.645%. The quantity of digestate was calculated on the basis of total N content, and 50% of the N requirements reported in the literature for silage maize was applied to the wheat stubble. Phylazonit MC was applied according to the instructions of the manufacturer, at a rate of 5 l/ha in 300 l water. Sowing was carried out three days after application. The following treatments were applied: digestate, digestate + Phylazonit MC, Phylazonit MC and control (untreated). The quantities of soil-borne heterotrophic, oligotrophic, spore-forming and free-living N 2-fixing bacteria and of microscopic fungi that could be cultured on selective nutrient media were analysed, and changes in the invertase and catalase activities in the soil were recorded in the 3rd and 9 th week after treatment. The number of r-strategists exhibited no deviation in samples taken in the 3rd week due to the addition of surplus nutrients. However, the quantity of K- and l-strategists and of free-living N2-fixing bacteria was highest in plots treated with digestate, and seasonal changes were also observed. By the end of September the number of colonies dropped in all the cultured groups, following the seasonal fluctuation. Treatment with digestate combined with Phylazonit MC led to a significant rise in the bacterium count compared with the control, while the number of microscopic fungi was smallest after treatment with digestate. Among the enzymes, the activity of catalase was highest three weeks after application and that of invertase after nine weeks, which could be attributed to their mode of action. It was proved that the success of treatment with Phylazonit MC was not related to the number of free-living N2-fixing bacteria, but was positively correlated with improvements in other soil properties. It could be concluded from the results that digestate enhanced the number and activity of soil microorganisms and stimulated the original microbe population of the soil, which, in addition to the nutrients added with the treatment, also made an indirect contribution to the increase in yield. The treatments investigated can thus be successfully included in sustainable agricultural practices, especially on sandy soils with low humus content. Table 1. Fresh mass of silage maize (t/ha) in the various treatments. (1) Treatment. a) Control; b) Phylazonit MC; c) Phylazonit MC + digestate; c) Digestate. (2) Fresh mass, t/ha; mean±SD. Note: a, b, c index: Significance groups in the Tukey's test (p<0.05). Table 2. Effect of treatment with Phylazonit MC and digestate on the number (log10) of colony-forming units (CFU) of selectively cultured microbe groups in the 3rd and 9th weeks after treatment. (1) Treatment: from top to bottom: control, Phylazonit MC, Phylazonit MC + digestate; digestate. (2) lg CFU mean±SD. (3) Heterotrophic. (4) Oligotrophic. (5) Spore-forming. (6) N2-fixing. (7) Microscopic fungi. A. In the 3rd week after treatment. B. In the 9th week after treatment. Note: See Table 1. Table 3. Changes in the activity of invertase and catalase in response to treatment with Phylazonit MC and digestate in the 3rd (16 Aug. 2006) and 9th (27 Sept. 2006) week after treatment. (1) See Table 1. (2) Activity, mean±SD. A. Invertase activity (mg glucose/g dry soil/4 h). B. Catalase activity (mg O2/g dry soil/h). Note: See Table 1. Table 4. Correlation coefficients (r) and significance levels (Szign.) for soil properties influencing the catalase enzyme in the 3rd week after treatment. (1) Soil property. a) Catalase activity (mg O2/g dry soil/h); b) No. of N2-fixing bacteria (lg CFU); c) Hydrolytic acidity, y1; d) Soil Mg content, mg/kg. (2) Catalase activity. (3) No. of N2-fixing bacteria. (4) Mg content. Fig. 1. Yields of silage maize grown in pots in 2005 and 2006, kg/pot. Fig. 2. Yields of silage maize in a small-plot experiment in 2006, t/ha.

Original languageHungarian
Pages (from-to)367-378
Number of pages12
JournalAgrokemia es Talajtan
Volume56
Issue number2
DOIs
Publication statusPublished - Dec 1 2007

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ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science

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