Effect of diets with different inclusion levels of distillers dried grain with solubles combined with lysine and methionine supplementation on the lipid peroxidation and glutathione status of chickens

Mónika Heincinger, K. Balogh, H. Fébel, Márta Erdélyi, M. Mézes

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To study the possible effects of different inclusion levels of distillers dried grain with solubles (DDGS) on the lipid peroxidation and glutathione redox status of chickens, 200 three-week-old Ross 308 cockerels were assigned to four treatment groups of 50 birds each. The groups were fed a control and three experimental, isocaloric and isonitrogenous grower diets containing 15, 20 and 25% DDGS, respectively, combined with lysine (Lys) and methionine (Met) supplementation until 6 weeks of age. It was found that DDGS inclusion increased the ether extract content of the diets which resulted in higher reduced glutathione (GSH) content and elevated glutathione peroxidase activity (GSHPx) in the liver. However, DDGS addition with Lys and Met supplementation did not influence the malondialdehyde content of the blood and the liver. The oleic acid proportion of the diet showed a close positive correlation with GSH content of the liver. A smaller ratio of methionine and cysteine in the diet with DDGS resulted in significantly higher liver GSH content. GSHPx activity increased parallel with the elevated GSH content of the liver homogenate, suggesting that the enzyme is activated by the actual supply of its co-substrate. In conclusion, the results show that DDGS, even at a high inclusion level combined with Lys and Met supplementation, has no initiative effect on lipid peroxidation in the blood and liver of broiler chickens.

Original languageEnglish
Pages (from-to)195-204
Number of pages10
JournalActa Veterinaria Hungarica
Volume59
Issue number2
DOIs
Publication statusPublished - Jun 1 2011

Fingerprint

distillers grains
Methionine
Lipid Peroxidation
Lysine
Glutathione
methionine
glutathione
Chickens
lysine
lipid peroxidation
chickens
Diet
liver
Liver
diet
Ross (chicken breed)
grower diets
cockerels
blood
Oleic Acid

Keywords

  • Chicken
  • DDGS
  • distillers dried grain with solubles
  • glutathione
  • glutathione peroxidase
  • malondialdehyde

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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title = "Effect of diets with different inclusion levels of distillers dried grain with solubles combined with lysine and methionine supplementation on the lipid peroxidation and glutathione status of chickens",
abstract = "To study the possible effects of different inclusion levels of distillers dried grain with solubles (DDGS) on the lipid peroxidation and glutathione redox status of chickens, 200 three-week-old Ross 308 cockerels were assigned to four treatment groups of 50 birds each. The groups were fed a control and three experimental, isocaloric and isonitrogenous grower diets containing 15, 20 and 25{\%} DDGS, respectively, combined with lysine (Lys) and methionine (Met) supplementation until 6 weeks of age. It was found that DDGS inclusion increased the ether extract content of the diets which resulted in higher reduced glutathione (GSH) content and elevated glutathione peroxidase activity (GSHPx) in the liver. However, DDGS addition with Lys and Met supplementation did not influence the malondialdehyde content of the blood and the liver. The oleic acid proportion of the diet showed a close positive correlation with GSH content of the liver. A smaller ratio of methionine and cysteine in the diet with DDGS resulted in significantly higher liver GSH content. GSHPx activity increased parallel with the elevated GSH content of the liver homogenate, suggesting that the enzyme is activated by the actual supply of its co-substrate. In conclusion, the results show that DDGS, even at a high inclusion level combined with Lys and Met supplementation, has no initiative effect on lipid peroxidation in the blood and liver of broiler chickens.",
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AU - Balogh, K.

AU - Fébel, H.

AU - Erdélyi, Márta

AU - Mézes, M.

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