Effect of dietary restriction on the degradation of proteins in senescent mouse liver parenchymal cells in culture

Akihito Ishigami, S. Goto

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We previously reported that the half-life of protein degradation in cells from old mice is about 50% longer than that in cells from young or middle-aged ones. In the present study we investigated the degradation rate of microinjected proteins (horseradish peroxidase (HRP) and ovalbumin (OVA)) and pulse-labeled proteins in hepatocytes from dietary-restricted old mice. Dietary restriction was initiated when mice were 23 months of age and performed in two steps (first 80% and then 60% of the ad libitum intake), the total period being 70 days. Hepatocytes were isolated from mice fed a restricted diet and fed ad libitum. The half-lives of HRP, OVA, and pulse-labelled proteins in the hepatocytes from mice fed a restricted diet were about 40% shorter than those in the cells from mice fed ad libitum. These values were close to those in the cells of young animals. These results are discussed in relation to our previous findings that a similar regimen reduces the percentage of heat-labile enzymes accumulated in tissues of aged animals.

Original languageEnglish
Pages (from-to)362-366
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume283
Issue number2
DOIs
Publication statusPublished - 1990

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Liver
Proteolysis
Cell Culture Techniques
Degradation
Ovalbumin
Horseradish Peroxidase
Nutrition
Hepatocytes
Animals
Proteins
Diet
Tissue
Half-Life
Enzymes
Hot Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effect of dietary restriction on the degradation of proteins in senescent mouse liver parenchymal cells in culture. / Ishigami, Akihito; Goto, S.

In: Archives of Biochemistry and Biophysics, Vol. 283, No. 2, 1990, p. 362-366.

Research output: Contribution to journalArticle

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