Effect of dexmedetomidine on the release of [3H]-noradrenaline from rat kidney cortex slices

Characterization of α2-adrenoceptor

Masahiko Taoda, Yushi U. Adachi, Yoshitaka Uchihashi, Kazuhiko Watanabe, Tetsuo Satoh, E. Vízi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The presynaptic modulation of [3H]-noradrenaline (NA) release from rat kidney cortex slices, a method used for the first time, was investigated. Rat kidney cortex slices were loaded with [3H]-NA and the release of radioactivity at rest and in response to field stimulation was determined. The α2-adrenoceptor agonist, dexmedetomidine inhibited the stimulation-evoked release of NA from kidney slices in a concentration-dependent manner, whereas α2-adrenoceptor antagonist CH-38083 (7,8-methyenedioxy-14-alpha-hydroxyalloberbane HCl), an α2-adrenoceptor antagonists, enhanced it. When dexmedetomidine and BRL-44408, a selective α2A antagonist, were added together, the effect of dexmedetomidine was significantly antagonized. In contrast, ARC-239 (2-(2,4-(o-piperazine-1-yl)-ethyl-4,4-dimethyl-1,3-(2H, 4H)disoguinolinedione chloride), a selective α2B-antagonist, had no effect on the release and failed to prevent the effect of dexmedetomidine. Prazosin, an α1- and α2B/C-adrenoceptor antagonist enhanced the release evoked by field stimulation. It is therefore suggested that there is a negative feedback modulation of NA release at the sympathetic innervation of kidney cortex, and dexmedetomidine, a clinically used anesthetic adjunct inhibits the release via activation of α2C-adrenoceptors.

Original languageEnglish
Pages (from-to)317-322
Number of pages6
JournalNeurochemistry International
Volume38
Issue number4
DOIs
Publication statusPublished - 2001

Fingerprint

Dexmedetomidine
Kidney Cortex
Adrenergic Receptors
Norepinephrine
Prazosin
Radioactivity
Anesthetics
Chlorides
Kidney

Keywords

  • α-Adrenoceptor
  • Dexmedetomidine
  • Rat kidney cortex slices

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Effect of dexmedetomidine on the release of [3H]-noradrenaline from rat kidney cortex slices : Characterization of α2-adrenoceptor. / Taoda, Masahiko; Adachi, Yushi U.; Uchihashi, Yoshitaka; Watanabe, Kazuhiko; Satoh, Tetsuo; Vízi, E.

In: Neurochemistry International, Vol. 38, No. 4, 2001, p. 317-322.

Research output: Contribution to journalArticle

Taoda, Masahiko ; Adachi, Yushi U. ; Uchihashi, Yoshitaka ; Watanabe, Kazuhiko ; Satoh, Tetsuo ; Vízi, E. / Effect of dexmedetomidine on the release of [3H]-noradrenaline from rat kidney cortex slices : Characterization of α2-adrenoceptor. In: Neurochemistry International. 2001 ; Vol. 38, No. 4. pp. 317-322.
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