Effect of detergents and sucrose derivatives on the modified protein determination of Lowry

D. Gaál, L. Holczinger

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effect on the protein determination method of Lowry, as modified by Hartree, of widely used detergents (sodium deoxycholate, sodium laurylsulphate, Triton X 100 and Triton WR 1339) and of sugar derivatives often used for preparing concentration gradients (sucrose, dextran, Ficoll and Metrizamide) was examined. Each of the compounds studied gave rise to a colored product in the test; however, the correlation between concentration and optical density was usually nonlinear. The majority of these substances, especially Ficoll, Metrizamide and dextran interfere with the procedure even at low concentrations, thus their elimination from the sample before protein determination is inevitable.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalActa Biochimica et Biophysica Academiae Scientiarum Hungaricae
Volume10
Issue number3
Publication statusPublished - 1975

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Metrizamide
Ficoll
Dextrans
Detergents
Sucrose
Deoxycholic Acid
Octoxynol
Proteins
Sodium
tyloxapol
dodecyl sulfate

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effect of detergents and sucrose derivatives on the modified protein determination of Lowry. / Gaál, D.; Holczinger, L.

In: Acta Biochimica et Biophysica Academiae Scientiarum Hungaricae, Vol. 10, No. 3, 1975, p. 201-206.

Research output: Contribution to journalArticle

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