Effect of cyclosporin A on the replication cycle of human immunodeficiency virus type 1 derived from H9 and Molt-4 producer cells

Carlton J. Briggs, J. Tőzsér, Stephen Oroszlan

Research output: Contribution to journalArticle

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Abstract

The effect of cyclosporin A (CsA) on the replication of human immunodeficiency virus type 1 (HIV-1) was studied. CsA treatment inhibited virus production in chronically infected H9 and Molt-4 cells. CsA treatment of HeLaCD4-LTR/β-gal cells or extracellular viruses also inhibited infection (IC50 1 μg/ml). The intracellular CsA-binding molecule cyclophilin A was detected in HIV-1 derived from chronically infected H9 cells, but it was present at a substantially lower level in HIV-1 derived from chronically infected Molt-4 cells. The low level of cyclophilin A in viral particles derived from Molt-4 cells correlated well with their substantially lower infectivity as assayed on HeLaCD4-LTR/β-gal cells. CsA treatment of infected cells showed a dose-dependent reduction of cyclophilin A incorporation into virions; the amount of cyclophilin A incorporation was found to be dependent on the producer cell type.

Original languageEnglish
Pages (from-to)2963-2967
Number of pages5
JournalJournal of General Virology
Volume77
Issue number12
Publication statusPublished - Dec 1996

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Cyclosporine
HIV-1
Cyclophilin A
Virion
Viruses
Inhibitory Concentration 50
Infection

ASJC Scopus subject areas

  • Virology
  • Immunology

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Effect of cyclosporin A on the replication cycle of human immunodeficiency virus type 1 derived from H9 and Molt-4 producer cells. / Briggs, Carlton J.; Tőzsér, J.; Oroszlan, Stephen.

In: Journal of General Virology, Vol. 77, No. 12, 12.1996, p. 2963-2967.

Research output: Contribution to journalArticle

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