The possible role of B protein, a postulated precursor of viral envelope proteins, was studied in Sindbis virus infected BHK cells. Although tryptic digestion of the B protein produces tryptic peptides of the viral envelope proteins it seems unlikely that B molecules accumulated in the cytoplasm of infected cells are subsequently cleaved into structural proteins. It is suggested that if B is really the precursor of E1 and PE2 viral envelope proteins, the cleavage takes place on the ribosomes during the synthesis of the second part of the B protein. Low concentrations of cycloheximide completely blocked the synthesis of B protein but did not inhibit the synthesis of viral structural proteins.
|Number of pages||4|
|Publication status||Published - Dec 1 1975|
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