Effect of Cycloartanes on Reversal of Multidrug Resistance and Apoptosis Induction on Mouse Lymphoma Cells

Ana Margarida Madureira, G. Spengler, A. Molnár, Andreas Varga, J. Molnár, Pedro M. Abreu, Maria José U Ferreira

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The ability of fifteen cycloartanes, isolated from Euphorbia species, to reverse multidrug resistance (MDR) and apoptosis induction in L5178Y mouse lymphoma cells, including its multidrug-resistant subline, was studied by flow cytometry. Reversion of MDR was investigated using a standard functional assay with rhodamine 123 as a fluorescent substrate analogue. For the evaluation of apoptosis, the cells were stained with FITC-labeled annexin V and propidium iodide. The majority of the compounds were able to reverse MDR of the tested human MDR1 gene-transfected mouse lymphoma cells. Some of the compounds were able to induce moderate apoptosis in the PAR cell line, but this effect was less effective on multidrug-resistant cells. The results indicate that cycloartanes can be substrates of ABC transporters, which might compete with certain anticancer chemotherapeutics.

Original languageEnglish
Pages (from-to)859-864
Number of pages6
JournalAnticancer Research
Volume24
Issue number2 B
Publication statusPublished - Mar 2004

Fingerprint

Multiple Drug Resistance
Lymphoma
Apoptosis
Euphorbia
Rhodamine 123
ATP-Binding Cassette Transporters
Propidium
Fluorescein-5-isothiocyanate
Annexin A5
Flow Cytometry
Cell Line
cycloartane
Genes

Keywords

  • Apoptosis induction
  • Cycloartane triterpenes
  • Euphorbia
  • Mouse lymphoma cell line
  • Multidrug resistance

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Effect of Cycloartanes on Reversal of Multidrug Resistance and Apoptosis Induction on Mouse Lymphoma Cells. / Madureira, Ana Margarida; Spengler, G.; Molnár, A.; Varga, Andreas; Molnár, J.; Abreu, Pedro M.; Ferreira, Maria José U.

In: Anticancer Research, Vol. 24, No. 2 B, 03.2004, p. 859-864.

Research output: Contribution to journalArticle

Madureira, Ana Margarida ; Spengler, G. ; Molnár, A. ; Varga, Andreas ; Molnár, J. ; Abreu, Pedro M. ; Ferreira, Maria José U. / Effect of Cycloartanes on Reversal of Multidrug Resistance and Apoptosis Induction on Mouse Lymphoma Cells. In: Anticancer Research. 2004 ; Vol. 24, No. 2 B. pp. 859-864.
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