Effect of conditioning apple shoots with meta-topolin on the morphogenic activity of in vitro leaves

J. Dobránszki, K. Magyar-Tábori, E. Jámbor-Benczúr, E. Kiss, J. Lazányi, T. Bubán

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The effects of a new type of aromatic cytokinin, meta-topolin, on the morphology and histology of apple leaves and its post-effects on the subsequent shoot regeneration from in vitro leaves were studied in cv. Royal Gala. The media applied for pre-treatment differed from each other in their cytokinin composition: medium No. 1 contained no cytokinin, No. 2 was supplemented with 0.5 mg l-1 benzyladenine, while Nos. 3-6 contained meta-topolin, the new type of cytokinin, in four concentrations (0.5-1.0-1.5-2.0 mg l -1). After a 3-week pre-treatment on these media shoot regeneration was induced on two test regeneration media containing thidiazuron (0.2 mg l -1) or benzyladenine (5.0 mg l-1). Irrespective of the pre-treatments, high regeneration (97-100%) was observed on all the regeneration media. However, the conditioning of apple shoots for three weeks on medium supplemented with meta-topolin in a concentration range between 0.5 and 1.5 mg l-1 caused a significant decrease in the rate of vitrified shoots (down to 13.4%) and increased the number of regenerated shoots per leaf segment significantly (up to 15.1). There was a positive correlation between the histological status and regeneration capacity of in vitro leaves. According to these results, meta-topolin, as a new source of cytokinin, could increase the morphogenic potential of apple leaves.

Original languageEnglish
Pages (from-to)117-126
Number of pages10
JournalActa Agronomica Hungarica
Volume50
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

cytokinins
apples
shoots
pretreatment
leaves
benzyladenine
histology
aromatic compounds
testing

Keywords

  • Anatomical structure
  • Conditioning
  • Meta-topolin
  • Organogenetic index

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Effect of conditioning apple shoots with meta-topolin on the morphogenic activity of in vitro leaves. / Dobránszki, J.; Magyar-Tábori, K.; Jámbor-Benczúr, E.; Kiss, E.; Lazányi, J.; Bubán, T.

In: Acta Agronomica Hungarica, Vol. 50, No. 2, 2002, p. 117-126.

Research output: Contribution to journalArticle

Dobránszki, J, Magyar-Tábori, K, Jámbor-Benczúr, E, Kiss, E, Lazányi, J & Bubán, T 2002, 'Effect of conditioning apple shoots with meta-topolin on the morphogenic activity of in vitro leaves', Acta Agronomica Hungarica, vol. 50, no. 2, pp. 117-126. https://doi.org/10.1556/AAgr.50.2002.2.1
Dobránszki, J. ; Magyar-Tábori, K. ; Jámbor-Benczúr, E. ; Kiss, E. ; Lazányi, J. ; Bubán, T. / Effect of conditioning apple shoots with meta-topolin on the morphogenic activity of in vitro leaves. In: Acta Agronomica Hungarica. 2002 ; Vol. 50, No. 2. pp. 117-126.
@article{970d0e8aa8fd409ba6cc139af55a1896,
title = "Effect of conditioning apple shoots with meta-topolin on the morphogenic activity of in vitro leaves",
abstract = "The effects of a new type of aromatic cytokinin, meta-topolin, on the morphology and histology of apple leaves and its post-effects on the subsequent shoot regeneration from in vitro leaves were studied in cv. Royal Gala. The media applied for pre-treatment differed from each other in their cytokinin composition: medium No. 1 contained no cytokinin, No. 2 was supplemented with 0.5 mg l-1 benzyladenine, while Nos. 3-6 contained meta-topolin, the new type of cytokinin, in four concentrations (0.5-1.0-1.5-2.0 mg l -1). After a 3-week pre-treatment on these media shoot regeneration was induced on two test regeneration media containing thidiazuron (0.2 mg l -1) or benzyladenine (5.0 mg l-1). Irrespective of the pre-treatments, high regeneration (97-100{\%}) was observed on all the regeneration media. However, the conditioning of apple shoots for three weeks on medium supplemented with meta-topolin in a concentration range between 0.5 and 1.5 mg l-1 caused a significant decrease in the rate of vitrified shoots (down to 13.4{\%}) and increased the number of regenerated shoots per leaf segment significantly (up to 15.1). There was a positive correlation between the histological status and regeneration capacity of in vitro leaves. According to these results, meta-topolin, as a new source of cytokinin, could increase the morphogenic potential of apple leaves.",
keywords = "Anatomical structure, Conditioning, Meta-topolin, Organogenetic index",
author = "J. Dobr{\'a}nszki and K. Magyar-T{\'a}bori and E. J{\'a}mbor-Bencz{\'u}r and E. Kiss and J. Laz{\'a}nyi and T. Bub{\'a}n",
year = "2002",
doi = "10.1556/AAgr.50.2002.2.1",
language = "English",
volume = "50",
pages = "117--126",
journal = "Acta Agronomica Hungarica",
issn = "0238-0161",
publisher = "Akademiai Kiado",
number = "2",

}

TY - JOUR

T1 - Effect of conditioning apple shoots with meta-topolin on the morphogenic activity of in vitro leaves

AU - Dobránszki, J.

AU - Magyar-Tábori, K.

AU - Jámbor-Benczúr, E.

AU - Kiss, E.

AU - Lazányi, J.

AU - Bubán, T.

PY - 2002

Y1 - 2002

N2 - The effects of a new type of aromatic cytokinin, meta-topolin, on the morphology and histology of apple leaves and its post-effects on the subsequent shoot regeneration from in vitro leaves were studied in cv. Royal Gala. The media applied for pre-treatment differed from each other in their cytokinin composition: medium No. 1 contained no cytokinin, No. 2 was supplemented with 0.5 mg l-1 benzyladenine, while Nos. 3-6 contained meta-topolin, the new type of cytokinin, in four concentrations (0.5-1.0-1.5-2.0 mg l -1). After a 3-week pre-treatment on these media shoot regeneration was induced on two test regeneration media containing thidiazuron (0.2 mg l -1) or benzyladenine (5.0 mg l-1). Irrespective of the pre-treatments, high regeneration (97-100%) was observed on all the regeneration media. However, the conditioning of apple shoots for three weeks on medium supplemented with meta-topolin in a concentration range between 0.5 and 1.5 mg l-1 caused a significant decrease in the rate of vitrified shoots (down to 13.4%) and increased the number of regenerated shoots per leaf segment significantly (up to 15.1). There was a positive correlation between the histological status and regeneration capacity of in vitro leaves. According to these results, meta-topolin, as a new source of cytokinin, could increase the morphogenic potential of apple leaves.

AB - The effects of a new type of aromatic cytokinin, meta-topolin, on the morphology and histology of apple leaves and its post-effects on the subsequent shoot regeneration from in vitro leaves were studied in cv. Royal Gala. The media applied for pre-treatment differed from each other in their cytokinin composition: medium No. 1 contained no cytokinin, No. 2 was supplemented with 0.5 mg l-1 benzyladenine, while Nos. 3-6 contained meta-topolin, the new type of cytokinin, in four concentrations (0.5-1.0-1.5-2.0 mg l -1). After a 3-week pre-treatment on these media shoot regeneration was induced on two test regeneration media containing thidiazuron (0.2 mg l -1) or benzyladenine (5.0 mg l-1). Irrespective of the pre-treatments, high regeneration (97-100%) was observed on all the regeneration media. However, the conditioning of apple shoots for three weeks on medium supplemented with meta-topolin in a concentration range between 0.5 and 1.5 mg l-1 caused a significant decrease in the rate of vitrified shoots (down to 13.4%) and increased the number of regenerated shoots per leaf segment significantly (up to 15.1). There was a positive correlation between the histological status and regeneration capacity of in vitro leaves. According to these results, meta-topolin, as a new source of cytokinin, could increase the morphogenic potential of apple leaves.

KW - Anatomical structure

KW - Conditioning

KW - Meta-topolin

KW - Organogenetic index

UR - http://www.scopus.com/inward/record.url?scp=33846528019&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846528019&partnerID=8YFLogxK

U2 - 10.1556/AAgr.50.2002.2.1

DO - 10.1556/AAgr.50.2002.2.1

M3 - Article

VL - 50

SP - 117

EP - 126

JO - Acta Agronomica Hungarica

JF - Acta Agronomica Hungarica

SN - 0238-0161

IS - 2

ER -