Effect of concanavalin A (Con-A) on the hormone production of the unicellular Tetrahymena and the immune cells of the rat. A comparative study

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Abstract

Tetrahymena populations were treated with 10-15 g ml -1 or 10-6 g ml-1 concanavalin-A (Con-A) in tryptone-yeast medium for 1 h. Rat peritoneal immune cells (mast cells, lymphocytes, monocyte-granulocyte group) were also treated with 10-6 g ml-1 Con-A, for 1 h. The cells' hormone (ACTH, histamine, serotonin, endorphin, triiodothyronine (T3)) content was measured by using immunocytochemistry and flow cytometry. The extremely low dose of Con-A universally and significantly elevated the hormone contents, while the result of higher dose was uncertain. In the immune cells, Con-A significantly decreased the ACTH level in each cell type and histamine level in mast cells. The results demonstrate the very high sensitivity of Tetrahymena receptors for a non-hormone (lectin) molecule, which can bind to the insulin receptors and mimics the effect of insulin. The results also show that Tetrahymena receptors are more sensitive to lower concentrations of molecules than to higher ones. The universal hormone-production stimulating effect of Con-A - which is observed in Tetrahymena - is specified in rat.

Original languageEnglish
Pages (from-to)578-581
Number of pages4
JournalCell Biochemistry and Function
Volume26
Issue number5
DOIs
Publication statusPublished - Jul 2008

Fingerprint

Tetrahymena
Concanavalin A
Rats
Cells
Hormones
Mast Cells
Adrenocorticotropic Hormone
Histamine
Endorphins
Molecules
Lymphocytes
Flow cytometry
Insulin Receptor
Triiodothyronine
Granulocytes
Lectins
Yeast
Monocytes
Serotonin
Flow Cytometry

Keywords

  • Concanavalin-A
  • Hormones
  • Insulin
  • Tetrahymena
  • White blood cells

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

Cite this

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abstract = "Tetrahymena populations were treated with 10-15 g ml -1 or 10-6 g ml-1 concanavalin-A (Con-A) in tryptone-yeast medium for 1 h. Rat peritoneal immune cells (mast cells, lymphocytes, monocyte-granulocyte group) were also treated with 10-6 g ml-1 Con-A, for 1 h. The cells' hormone (ACTH, histamine, serotonin, endorphin, triiodothyronine (T3)) content was measured by using immunocytochemistry and flow cytometry. The extremely low dose of Con-A universally and significantly elevated the hormone contents, while the result of higher dose was uncertain. In the immune cells, Con-A significantly decreased the ACTH level in each cell type and histamine level in mast cells. The results demonstrate the very high sensitivity of Tetrahymena receptors for a non-hormone (lectin) molecule, which can bind to the insulin receptors and mimics the effect of insulin. The results also show that Tetrahymena receptors are more sensitive to lower concentrations of molecules than to higher ones. The universal hormone-production stimulating effect of Con-A - which is observed in Tetrahymena - is specified in rat.",
keywords = "Concanavalin-A, Hormones, Insulin, Tetrahymena, White blood cells",
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