Effect of combined changes in culture medium and incubation conditions on the regeneration from immature embryos of elite varieties of winter wheat

Cecília Tamás, Péter Suzcs, Mariann Rakszegi, László Tamás, Zoltán Bedő

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14 Citations (Scopus)

Abstract

In this study, tissue culture method for plant regeneration from immature embryos of elite Hungarian winter wheat varieties was established. The influence of the growth regulators and the concentration of macroelements in the regeneration medium and of the incubation temperature and light intensity on regeneration frequency were investigated. The most noticeable effect on regeneration frequency was achieved by simultaneously reducing both the incubation temperature to 23°C and the concentration of macroelements in the regeneration medium to half-strength. This modification increased the average regeneration frequency from about 10-78%. Changes in the light intensity and temperature gave an average plant regeneration frequency of 83%.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalPlant Cell, Tissue and Organ Culture
Volume79
Issue number1
DOIs
Publication statusPublished - Oct 2004

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Keywords

  • Triticum aestivum L.
  • immature embryo
  • in vitro culture
  • light
  • shoot regeneration
  • temperature

ASJC Scopus subject areas

  • Horticulture

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