Effect of column temperature and nitrogen flow rate on the separation of cis-permethrinic acid isomers on a (2,3,6-Tri-O-methyl)-β-cyclodextrin coated capillary column

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Abstract

The separation efficiency of a capillary column coated with (2,3,6-tri-O-methyl)-β-cyclodextrin was determined at the temperature and Bow rate ranges of 80-120°C and 0.3-1.5 mL/min respectively using (+) and (-) cis-permethrinic acid methylesters as test compounds. The dependence of the retention differences, sum of peak widths and separation factor on the temperature and flow rate was calculated with step regression analysis. The column exhibited acceptable isomer separation capacity at each temperature and flow rate proving the excellent separation power of the cyclodextrin coating. The differences between the retention times of isomers depended on the logarithm and on the reciprocal value of temperature, the effect of flow rate was negligible. The sum of their peak widths depended reciprocally on the temperature and logarithmically on the flow rate, the impact of temperature being higher than that of flow rate. The significant impact of the reciprocal value of temperature can be explained by the inclusion complex formation between cyclodextrin and cis-permethrinic acid methylester isomers. The resolution depended on the square of column temperature and flow rate, indicating local optimums at each temperature and flow rate.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalBiomedical Chromatography
Volume9
Issue number3
DOIs
Publication statusPublished - 1995

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NSC 153174
Cyclodextrins
Isomers
Nitrogen
Flow rate
Temperature

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

Cite this

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title = "Effect of column temperature and nitrogen flow rate on the separation of cis-permethrinic acid isomers on a (2,3,6-Tri-O-methyl)-β-cyclodextrin coated capillary column",
abstract = "The separation efficiency of a capillary column coated with (2,3,6-tri-O-methyl)-β-cyclodextrin was determined at the temperature and Bow rate ranges of 80-120°C and 0.3-1.5 mL/min respectively using (+) and (-) cis-permethrinic acid methylesters as test compounds. The dependence of the retention differences, sum of peak widths and separation factor on the temperature and flow rate was calculated with step regression analysis. The column exhibited acceptable isomer separation capacity at each temperature and flow rate proving the excellent separation power of the cyclodextrin coating. The differences between the retention times of isomers depended on the logarithm and on the reciprocal value of temperature, the effect of flow rate was negligible. The sum of their peak widths depended reciprocally on the temperature and logarithmically on the flow rate, the impact of temperature being higher than that of flow rate. The significant impact of the reciprocal value of temperature can be explained by the inclusion complex formation between cyclodextrin and cis-permethrinic acid methylester isomers. The resolution depended on the square of column temperature and flow rate, indicating local optimums at each temperature and flow rate.",
author = "K. Payer and E. Forg{\'a}cs and T. Cserh{\'a}ti",
year = "1995",
doi = "10.1002/bmc.1130090305",
language = "English",
volume = "9",
pages = "135--139",
journal = "Biomedical Chromatography",
issn = "0269-3879",
publisher = "John Wiley and Sons Ltd",
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TY - JOUR

T1 - Effect of column temperature and nitrogen flow rate on the separation of cis-permethrinic acid isomers on a (2,3,6-Tri-O-methyl)-β-cyclodextrin coated capillary column

AU - Payer, K.

AU - Forgács, E.

AU - Cserháti, T.

PY - 1995

Y1 - 1995

N2 - The separation efficiency of a capillary column coated with (2,3,6-tri-O-methyl)-β-cyclodextrin was determined at the temperature and Bow rate ranges of 80-120°C and 0.3-1.5 mL/min respectively using (+) and (-) cis-permethrinic acid methylesters as test compounds. The dependence of the retention differences, sum of peak widths and separation factor on the temperature and flow rate was calculated with step regression analysis. The column exhibited acceptable isomer separation capacity at each temperature and flow rate proving the excellent separation power of the cyclodextrin coating. The differences between the retention times of isomers depended on the logarithm and on the reciprocal value of temperature, the effect of flow rate was negligible. The sum of their peak widths depended reciprocally on the temperature and logarithmically on the flow rate, the impact of temperature being higher than that of flow rate. The significant impact of the reciprocal value of temperature can be explained by the inclusion complex formation between cyclodextrin and cis-permethrinic acid methylester isomers. The resolution depended on the square of column temperature and flow rate, indicating local optimums at each temperature and flow rate.

AB - The separation efficiency of a capillary column coated with (2,3,6-tri-O-methyl)-β-cyclodextrin was determined at the temperature and Bow rate ranges of 80-120°C and 0.3-1.5 mL/min respectively using (+) and (-) cis-permethrinic acid methylesters as test compounds. The dependence of the retention differences, sum of peak widths and separation factor on the temperature and flow rate was calculated with step regression analysis. The column exhibited acceptable isomer separation capacity at each temperature and flow rate proving the excellent separation power of the cyclodextrin coating. The differences between the retention times of isomers depended on the logarithm and on the reciprocal value of temperature, the effect of flow rate was negligible. The sum of their peak widths depended reciprocally on the temperature and logarithmically on the flow rate, the impact of temperature being higher than that of flow rate. The significant impact of the reciprocal value of temperature can be explained by the inclusion complex formation between cyclodextrin and cis-permethrinic acid methylester isomers. The resolution depended on the square of column temperature and flow rate, indicating local optimums at each temperature and flow rate.

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