Effect of cholestyramine-induced bile acid depletion on the hepatobiliary transport of cholephilic organic anions in rats

Z. Gregus, E. Fischer, F. Varga

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Abstract

Four hours after oral administration of cholestyramine (1.0 g/kg) the biliary output of bile acids decreased to 21 per cent of the control value. The biliary excretion of indocyanine green (ICG; 15-60 μmol/kg i.v.), rose bengal (RB; 15-60 μmol/kg i.v.), bromsulphthalein (BSP; 15-60 μmol/kg i.v.), bromcresol green (BCG;15-60 μmol/kg i.v.) and eosine (EO; 30-120 μmol/kg i.v.) was considerably depressed by cholestyramine pretreatment. The extent and duration of the reduction in the biliary excretion of organic anions in response to bile acid depletion were found to increase with their doses. In contrast, the biliary excretion rates of bromsulphthalein-glutathione conjugate (BSP-GSH; 60-240 μmol/kg i.v.) and amaranth (AM; 60-240 μmol/kg i.v.) remained unchanged following bile acid depletion. The hepatic uptake of the cholephilic agents investigated was not affected by bile acid depletion. It is concluded that the biliary excretion rates of ICG, RB, BSP, BCG and EO are dependent on the excretion rates of endogenous bile acids. The hapatic transport rates of BSP-GSH and AM, however, seem to be relatively insensitive to the biliary output of endogenous bile salts.

Original languageEnglish
Pages (from-to)311-322
Number of pages12
JournalArchives Internationales de Pharmacodynamie et de Therapie
Volume245
Issue number2
Publication statusPublished - 1980

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Cholestyramine Resin
Bile Acids and Salts
Anions
Mycobacterium bovis
Bromcresol Green
Rose Bengal
Indocyanine Green
Eosine Yellowish-(YS)
Glutathione
Oral Administration
Hepatobiliary Elimination
Liver

ASJC Scopus subject areas

  • Pharmacology

Cite this

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title = "Effect of cholestyramine-induced bile acid depletion on the hepatobiliary transport of cholephilic organic anions in rats",
abstract = "Four hours after oral administration of cholestyramine (1.0 g/kg) the biliary output of bile acids decreased to 21 per cent of the control value. The biliary excretion of indocyanine green (ICG; 15-60 μmol/kg i.v.), rose bengal (RB; 15-60 μmol/kg i.v.), bromsulphthalein (BSP; 15-60 μmol/kg i.v.), bromcresol green (BCG;15-60 μmol/kg i.v.) and eosine (EO; 30-120 μmol/kg i.v.) was considerably depressed by cholestyramine pretreatment. The extent and duration of the reduction in the biliary excretion of organic anions in response to bile acid depletion were found to increase with their doses. In contrast, the biliary excretion rates of bromsulphthalein-glutathione conjugate (BSP-GSH; 60-240 μmol/kg i.v.) and amaranth (AM; 60-240 μmol/kg i.v.) remained unchanged following bile acid depletion. The hepatic uptake of the cholephilic agents investigated was not affected by bile acid depletion. It is concluded that the biliary excretion rates of ICG, RB, BSP, BCG and EO are dependent on the excretion rates of endogenous bile acids. The hapatic transport rates of BSP-GSH and AM, however, seem to be relatively insensitive to the biliary output of endogenous bile salts.",
author = "Z. Gregus and E. Fischer and F. Varga",
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T1 - Effect of cholestyramine-induced bile acid depletion on the hepatobiliary transport of cholephilic organic anions in rats

AU - Gregus, Z.

AU - Fischer, E.

AU - Varga, F.

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N2 - Four hours after oral administration of cholestyramine (1.0 g/kg) the biliary output of bile acids decreased to 21 per cent of the control value. The biliary excretion of indocyanine green (ICG; 15-60 μmol/kg i.v.), rose bengal (RB; 15-60 μmol/kg i.v.), bromsulphthalein (BSP; 15-60 μmol/kg i.v.), bromcresol green (BCG;15-60 μmol/kg i.v.) and eosine (EO; 30-120 μmol/kg i.v.) was considerably depressed by cholestyramine pretreatment. The extent and duration of the reduction in the biliary excretion of organic anions in response to bile acid depletion were found to increase with their doses. In contrast, the biliary excretion rates of bromsulphthalein-glutathione conjugate (BSP-GSH; 60-240 μmol/kg i.v.) and amaranth (AM; 60-240 μmol/kg i.v.) remained unchanged following bile acid depletion. The hepatic uptake of the cholephilic agents investigated was not affected by bile acid depletion. It is concluded that the biliary excretion rates of ICG, RB, BSP, BCG and EO are dependent on the excretion rates of endogenous bile acids. The hapatic transport rates of BSP-GSH and AM, however, seem to be relatively insensitive to the biliary output of endogenous bile salts.

AB - Four hours after oral administration of cholestyramine (1.0 g/kg) the biliary output of bile acids decreased to 21 per cent of the control value. The biliary excretion of indocyanine green (ICG; 15-60 μmol/kg i.v.), rose bengal (RB; 15-60 μmol/kg i.v.), bromsulphthalein (BSP; 15-60 μmol/kg i.v.), bromcresol green (BCG;15-60 μmol/kg i.v.) and eosine (EO; 30-120 μmol/kg i.v.) was considerably depressed by cholestyramine pretreatment. The extent and duration of the reduction in the biliary excretion of organic anions in response to bile acid depletion were found to increase with their doses. In contrast, the biliary excretion rates of bromsulphthalein-glutathione conjugate (BSP-GSH; 60-240 μmol/kg i.v.) and amaranth (AM; 60-240 μmol/kg i.v.) remained unchanged following bile acid depletion. The hepatic uptake of the cholephilic agents investigated was not affected by bile acid depletion. It is concluded that the biliary excretion rates of ICG, RB, BSP, BCG and EO are dependent on the excretion rates of endogenous bile acids. The hapatic transport rates of BSP-GSH and AM, however, seem to be relatively insensitive to the biliary output of endogenous bile salts.

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