Effect of chlorophenoxyacetic acid herbicides on glycine conjugation of benzoic acid

Z. Gregus, É Halászi, C. D. Klaassen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

1. 2,4-Dichlorophenoxyacetic acid (2,4-D) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) (0.1-0.5 mmol/kg i.p.) delayed the disappearance of injected benzoate from blood and diminished the urinary excretion of the formed benzoylglycine, but elevated the blood levels of benzoylglycine in rat, suggesting that these herbicides interfere with both the formation and the renal transport of benzoylglycine. 2. Inhibition of the renal excretion of benzoylglycine by 2,4-D or 2,4,5-T (0.5 mmol/kg i.p.) was directly demonstrated in rat injected with benzoylglycine. 3. Inhibition of benzoylglycine formation from benzoic acid by 2,4-D or 2,4,5-T (0.5 mmol/kg i.p.) was directly demonstrated in renal pedicles-ligated rats injected with benzoate. 4. Neither 2,4-D nor 2,4,5-T influenced the hepatic concentrations of ATP, coenzyme A (CoA) or glycine; therefore, it is unlikely that they inhibit glycine conjugation of benzoic acid by diminishing the availability of co-substrates. 5. Although the chlorophenoxyacetic acids did not appear to be a substrate for the mirochondrial acyl-CoA synthetases, both 2,4-D and 2,4,5-T diminished the activity of benzyol-CoA synthetase (but not that of benzol-CoA glycine N-acyltransferase) in solubilized hepatic mitochondria. These findings suggest that 2,4-D and 2,4,5-T impair benzoylglycine formation in rat by inhibiting benzoyl-CoA synthetase.

Original languageEnglish
Pages (from-to)547-559
Number of pages13
JournalXenobiotica
Volume29
Issue number6
DOIs
Publication statusPublished - 1999

Fingerprint

2,4,5-Trichlorophenoxyacetic Acid
2,4-Dichlorophenoxyacetic Acid
Benzoic Acid
Herbicides
Glycine
Coenzyme A Ligases
Acids
Rats
Benzoates
glycine acyltransferase
Coenzyme A
Blood
Kidney
Mitochondria
Liver
Substrates
hippuric acid
Benzene
Adenosine Triphosphate
Availability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Effect of chlorophenoxyacetic acid herbicides on glycine conjugation of benzoic acid. / Gregus, Z.; Halászi, É; Klaassen, C. D.

In: Xenobiotica, Vol. 29, No. 6, 1999, p. 547-559.

Research output: Contribution to journalArticle

Gregus, Z. ; Halászi, É ; Klaassen, C. D. / Effect of chlorophenoxyacetic acid herbicides on glycine conjugation of benzoic acid. In: Xenobiotica. 1999 ; Vol. 29, No. 6. pp. 547-559.
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