Effect of Chilling on Assimilatory Sulfate Reduction and Glutathione Synthesis in Maize

Monika Brunner, Gábor Kocsy, Adrian Rftegsegger, Daniel Schmutz, Christian Brunold

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The contents of cysteine, γ-glutamylcysteine and glutathione, as well as the activity of enzymes of assimilatory sulfate reduction and glutathione synthesis in second leaves and root tips of maize seedlings (Zea mays L. cv. LG 11) cultivated at low temperatures, were compared with controls grown at 25 °C. In vitro nitrate reductase (EC activity was measured for comparison. Compared with the controls, adenosine 5'-phosphosulfate sulf otransf erase activity was 3.5- and 5.5-fold higher at 12 °C in second leaves and root tips, respectively. After 3 days of growth, the activity of y,-glutamylcysteine synthetase (EC and glutathione synthetase (EC in the second leaves was also significantly higher at 12 °C compared with 25 °C. Consistent with these enzyme activities, there was an increase in cysteine and glutathione contents both in second leaves and root tips after 3 days of growth at 12 °C. Nitrate reductase activity of second leaves and root tips was not affected by growth at 12 °C, indicating that the increase in adenosine 5'-phosphosulfate sulfotransferase activity and of the enzymes of glutathione synthesis was specific. Our results demonstrate that the increased activity of a key enzyme of assimilatory sulfate reduction and of the enzymes of glutathione synthesis contribute to the increased glutathione levels measured at 12 °C.

Original languageEnglish
Pages (from-to)743-747
Number of pages5
JournalJournal of Plant Physiology
Issue number5-6
Publication statusPublished - Jan 1 1995



  • 2-(cyclohexylamino)-ethanesulfonic acid
  • APS
  • APSSTase
  • CHES
  • FW
  • GSH
  • NR
  • RH
  • Zea mays
  • adenosine 5' phosphosulfate sulfotransferase
  • adenosine 5'-phosphosulfate
  • adenosine 5'-phosphosulfate sulfotransferase
  • chilling
  • fresh weight
  • glutathione
  • glutathione
  • glutathione synthetase
  • nitrate reductase
  • nitrate reductase
  • relative humidity
  • yEC
  • γ-glutamylcysteine
  • γ-glutamylcysteine synthetase

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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