Effect of catalytic hydrogenation of Tetrahymena ciliary phospholipid fatty acids on ciliary phospholipase A activity

I. Horváth, L. Vígh, T. Páli, Guy A. Thompson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Detached Tetrahymena cilia were treated for increasing periods of time with the homogeneous hydrogenation catalyst palladium di(sodium alizarine monosulphonate). This caused a 4-70% reduction in the number of double bonds in phospholipid-bound fatty acids and a concurrent decrease in membrane fluidity as detected by ESR measurements. Ciliary phospholipase A activity was markedly inhibited when as little as 13% of the fatty acid double bonds had been hydrogenated, suggesting that the enzyme activity is very sensitive to changes in membrane fluidity.

Original languageEnglish
Pages (from-to)409-412
Number of pages4
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1002
Issue number3
DOIs
Publication statusPublished - Apr 26 1989

Fingerprint

Tetrahymena
Membrane Fluidity
Hydrogenation
Fluidity
Phospholipases A
Phospholipids
Fatty Acids
Membranes
Cilia
Enzyme activity
Paramagnetic resonance
Catalysts
Enzymes
palladium sulfonated alizarine

Keywords

  • (Tetrahymena)
  • Phospholipase A
  • Phospholipid retailoring
  • Plasma membrane

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology
  • Medicine(all)

Cite this

@article{76ec3bbb36da4b18b59c26727d1c79a9,
title = "Effect of catalytic hydrogenation of Tetrahymena ciliary phospholipid fatty acids on ciliary phospholipase A activity",
abstract = "Detached Tetrahymena cilia were treated for increasing periods of time with the homogeneous hydrogenation catalyst palladium di(sodium alizarine monosulphonate). This caused a 4-70{\%} reduction in the number of double bonds in phospholipid-bound fatty acids and a concurrent decrease in membrane fluidity as detected by ESR measurements. Ciliary phospholipase A activity was markedly inhibited when as little as 13{\%} of the fatty acid double bonds had been hydrogenated, suggesting that the enzyme activity is very sensitive to changes in membrane fluidity.",
keywords = "(Tetrahymena), Phospholipase A, Phospholipid retailoring, Plasma membrane",
author = "I. Horv{\'a}th and L. V{\'i}gh and T. P{\'a}li and Thompson, {Guy A.}",
year = "1989",
month = "4",
day = "26",
doi = "10.1016/0005-2760(89)90359-7",
language = "English",
volume = "1002",
pages = "409--412",
journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",
issn = "1388-1981",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Effect of catalytic hydrogenation of Tetrahymena ciliary phospholipid fatty acids on ciliary phospholipase A activity

AU - Horváth, I.

AU - Vígh, L.

AU - Páli, T.

AU - Thompson, Guy A.

PY - 1989/4/26

Y1 - 1989/4/26

N2 - Detached Tetrahymena cilia were treated for increasing periods of time with the homogeneous hydrogenation catalyst palladium di(sodium alizarine monosulphonate). This caused a 4-70% reduction in the number of double bonds in phospholipid-bound fatty acids and a concurrent decrease in membrane fluidity as detected by ESR measurements. Ciliary phospholipase A activity was markedly inhibited when as little as 13% of the fatty acid double bonds had been hydrogenated, suggesting that the enzyme activity is very sensitive to changes in membrane fluidity.

AB - Detached Tetrahymena cilia were treated for increasing periods of time with the homogeneous hydrogenation catalyst palladium di(sodium alizarine monosulphonate). This caused a 4-70% reduction in the number of double bonds in phospholipid-bound fatty acids and a concurrent decrease in membrane fluidity as detected by ESR measurements. Ciliary phospholipase A activity was markedly inhibited when as little as 13% of the fatty acid double bonds had been hydrogenated, suggesting that the enzyme activity is very sensitive to changes in membrane fluidity.

KW - (Tetrahymena)

KW - Phospholipase A

KW - Phospholipid retailoring

KW - Plasma membrane

UR - http://www.scopus.com/inward/record.url?scp=0024978919&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024978919&partnerID=8YFLogxK

U2 - 10.1016/0005-2760(89)90359-7

DO - 10.1016/0005-2760(89)90359-7

M3 - Article

C2 - 2713391

AN - SCOPUS:0024978919

VL - 1002

SP - 409

EP - 412

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 3

ER -