Effect of cadmium on the relationship between replicative and repair DNA synthesis in synchronized CHO cells

Gaspar Banfalvi, Neil Littlefield, Bruce Hass Marina Mikhailova, Ildiko Csuka, Edit Szepessy, Ming Wu Chou

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Repair and replicative DNA synthesis were measured at different stages of the cell cycle in control and cadmium-treated Chinese hamster ovary (CHO-K1) cells. Cells were synchronized by counterflow centrifugal elutriation. Elutriation resulted in five repair and four replication subphases. On Cd treatment, repair synthesis was elevated in certain subphases. Replicative subphases were suppressed by Cd treatment, with some of the peaks almost invisible. The number of spontaneous strand breaks measured by random oligonucleotide primed synthesis assay showed a cell-cycle-dependent fluctuation in control cells and was greatly increased after Cd treatment throughout the S phase. Elevated levels of the oxidative DNA damage product, 8-oxodeoxyguanosine, were observed after Cd treatment, with the highest level in early S phase, which gradually declined as damaged cells progressed through the cell cycle.

Original languageEnglish
Pages (from-to)6580-6585
Number of pages6
JournalEuropean Journal of Biochemistry
Volume267
Issue number22
DOIs
Publication statusPublished - Nov 25 2000

Keywords

  • 8-oxodeoxyguanosine
  • Elutriation
  • Permeable cells
  • Strand breaks
  • Synchronization

ASJC Scopus subject areas

  • Biochemistry

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