Effect of cadmium on iron uptake in cucumber roots

A Mössbauer-spectroscopic study

Krisztina Kovács, E. Kuzmann, A. Vértes, László Lévai, E. Cseh, F. Fodor

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The uptake and accumulation of iron in cucumber roots exposed to cadmium were investigated with Fe sufficient and deficient cucumber plants using Mössbauer spectroscopy, Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and ferric chelate reductase activity measurements. Both Fe sufficient and Fe deficient plants were applied. In the case of Fe sufficient cucumber roots grown in nutrient solution with 10 μM Cd no changes were found in the occurrence of Fe species (mostly hydrous ferric oxides and ferric-carboxylate complexes) compared to the control where no Cd was added. In the Fe deficient roots pretreated with 0, 0.1, 1, 10 and 100 μM Cd for 3 h then supplied also with 0.5 mM 57Fe-citrate for 30 min, FeII was identified in a hexaaqua complex form. The relative amount of FeII was decreasing simultaneously with increasing Cd concentration, while the relative occurrence of FeIII species and total Fe concentration were increasing. The results support the inhibitory effect of Cd on Fe-chelate reduction. Although the reductase activity at 10 and 100 μM Cd treatment was lower than in the iron sufficient control plants, FeII could be identified by Mössbauer spectroscopy whereas in the Fe sufficient control, this form was below detection limit. These data demonstrate that the influx and the reoxidation of FeII was decreased by Cd, consequently, they refer to the competition of Cd2+ and Fe2+ during the membrane transport and the inhibition of the reoxidation process.

Original languageEnglish
Pages (from-to)49-56
Number of pages8
JournalPlant and Soil
Volume327
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

cucumbers
cadmium
chelate
chelates
iron
spectroscopy
atomic absorption spectrometry
citrates
nutrient solutions
detection limit
mass spectrometry
oxide
membrane
plasma
nutrient
effect
ferric oxide
detection

Keywords

  • Cadmium
  • Fe reductase activity
  • Fe-citrate
  • Iron
  • Mössbauer spectroscopy
  • Strategy I

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

Cite this

Effect of cadmium on iron uptake in cucumber roots : A Mössbauer-spectroscopic study. / Kovács, Krisztina; Kuzmann, E.; Vértes, A.; Lévai, László; Cseh, E.; Fodor, F.

In: Plant and Soil, Vol. 327, No. 1, 01.2010, p. 49-56.

Research output: Contribution to journalArticle

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AB - The uptake and accumulation of iron in cucumber roots exposed to cadmium were investigated with Fe sufficient and deficient cucumber plants using Mössbauer spectroscopy, Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and ferric chelate reductase activity measurements. Both Fe sufficient and Fe deficient plants were applied. In the case of Fe sufficient cucumber roots grown in nutrient solution with 10 μM Cd no changes were found in the occurrence of Fe species (mostly hydrous ferric oxides and ferric-carboxylate complexes) compared to the control where no Cd was added. In the Fe deficient roots pretreated with 0, 0.1, 1, 10 and 100 μM Cd for 3 h then supplied also with 0.5 mM 57Fe-citrate for 30 min, FeII was identified in a hexaaqua complex form. The relative amount of FeII was decreasing simultaneously with increasing Cd concentration, while the relative occurrence of FeIII species and total Fe concentration were increasing. The results support the inhibitory effect of Cd on Fe-chelate reduction. Although the reductase activity at 10 and 100 μM Cd treatment was lower than in the iron sufficient control plants, FeII could be identified by Mössbauer spectroscopy whereas in the Fe sufficient control, this form was below detection limit. These data demonstrate that the influx and the reoxidation of FeII was decreased by Cd, consequently, they refer to the competition of Cd2+ and Fe2+ during the membrane transport and the inhibition of the reoxidation process.

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