Kadmium és titán-aszkorbát okozta stressz hatása a búzanövények biológiailag aktív komponenseire

Translated title of the contribution: Effect of cadmium and titanium-ascorbatc stress on biologically active compounds in wheat seedlings

Leskó Kornélia, E. Stefanovits-Banyai, Pais István, L. Simon-Sarkadi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The harmful processes caused by heavy metals are serious problems not only for useful plants but, if they enter the food chain, for consumers as well. This is why it is essential to clarify biochemical processes. The effect of cadmium and combined cadmium and titanium-ascorbatc was examined on wheat seedlings (Triticum aestvim L.). The one-wcek-old hydroponically-grown seedlings of wheat were exposed to Cd2+ (10-7 M, 10-3 M) and Cd2+ (10-7 M, 10-3 M) + titanium-ascorbate (5 ng/cm3) stresses. The stress effects were followed by changes in the free amino acid and polyaminc contents and the peroxidasc enzyme activity in roots and shoots. The free amino acid content was determined by ion-exchange liquid chromatography. The polyaminc content was identified by OPLC (over-prcssured layer chromatography). Pcroxydase activity (U/mg protein) was measured by the spectrophotomctric method. The results showed significant differences between the two plant organs (root and shoot) with respect to the biochemical response to the applied stress. The total free amino acid content of the shoot samples was higher than that of the roots. Cadmium treatment at the higher concentration caused the highest accumulation of total amino acid content in both the shoots and roots. Among the amino acids the thrconinc, scrinc+ (Scr+Asn+Gln), glutamic acid, proline, alanine, valinc, isolcucinc, leucine, histidinc, lysine and arginine concentrations showed the most significant changes. The different effects of cadmium and the combined cadmium+titanium-ascorbate treatment can be characterised based on the typical accumulation of polyamines in the two organs of wheat seedlings. Agmatinc accumulation was observed in the control shoot sample while in the shoot sample treated with Cd2+ (10-7 M) combined with titanium-ascorbate, putrcscinc tended to accumulate. In the roots only putrcscine increased significantly. Peroxidasc activity was more significant in the root samples than in the shoot samples. The highest peroxidasc activity was measured in the case of the Cd2+ (10-3 M) treatment in the roots. Titanium-ascorbatc treatment seemed to reduce the negative effects of heavy metal pollution in wheat seedlings. On the basis of the results it can be stated that the heavy metal stress caused typical biochemical changes in wheat seedlings. Further research is needed to find out the relationship between the heavy metal stress and the changes in free amino acid and polyamine contents as well as in peroxidase activity.

Original languageHungarian
Pages (from-to)71-81
Number of pages11
JournalNovenytermeles
Volume50
Issue number1
Publication statusPublished - 2001

Fingerprint

titanium
cadmium
wheat
shoots
seedlings
free amino acids
heavy metals
polyamines
sampling
amino acids
Triticum
ion exchange chromatography
plant organs
glutamic acid
food chain
alanine
liquid chromatography
leucine
arginine
proline

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Kadmium és titán-aszkorbát okozta stressz hatása a búzanövények biológiailag aktív komponenseire. / Kornélia, Leskó; Stefanovits-Banyai, E.; István, Pais; Simon-Sarkadi, L.

In: Novenytermeles, Vol. 50, No. 1, 2001, p. 71-81.

Research output: Contribution to journalArticle

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