Effect of cadmium and magnesium on free amino acids, polyamines and peroxidase enzyme activity in wheat seedlings

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Abstract

The effect of cadmium and combined cadmium and magnesium was examined on wheat seedlings (Triticum aestivum L. cv. Alföld-90). The one-week-old hydroponically-grown seedlings of wheat were exposed to Cd2+(10-7 M, 10-3 M) and Cd2+(10-7 M, 10-3 M) + 1% MgCl2 stresses. The stress effects were followed by changes in free amino acid, polyamine contents and peroxidase enzyme activity in roots and shoots. Free amino acid content was determined by ion-exchange liquid chromatography. Polyamine content was identified by OPLC (overpressured layer chromatography). Peroxidase activity (U/mg protein) was measured by spectrophotometric method. The results showed significant differences between the two organs of the plant (root and shoot) with respect to the biochemical response tO the applied stress. The cadmium stress caused the accumulation of proline in both shoots and roots. Magnesium reduced the proline content in the samples treated with cadmium. The magnesium treatment seemed to reduce the negative effects of cadmium in wheat seedlings.

Original languageEnglish
Pages (from-to)103-110
Number of pages8
JournalCereal Research Communications
Volume30
Issue number1-2
Publication statusPublished - 2002

Fingerprint

Polyamines
polyamines
Cadmium
Seedlings
free amino acids
Magnesium
Peroxidase
Triticum
cadmium
magnesium
peroxidase
enzyme activity
Amino Acids
wheat
seedlings
Enzymes
Proline
shoots
proline
Plant Shoots

Keywords

  • Cadmium
  • Free amino acid
  • Magnesium
  • Peroxidase
  • Polyamine
  • Proline
  • Stress
  • Wheat

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "Effect of cadmium and magnesium on free amino acids, polyamines and peroxidase enzyme activity in wheat seedlings",
abstract = "The effect of cadmium and combined cadmium and magnesium was examined on wheat seedlings (Triticum aestivum L. cv. Alf{\"o}ld-90). The one-week-old hydroponically-grown seedlings of wheat were exposed to Cd2+(10-7 M, 10-3 M) and Cd2+(10-7 M, 10-3 M) + 1{\%} MgCl2 stresses. The stress effects were followed by changes in free amino acid, polyamine contents and peroxidase enzyme activity in roots and shoots. Free amino acid content was determined by ion-exchange liquid chromatography. Polyamine content was identified by OPLC (overpressured layer chromatography). Peroxidase activity (U/mg protein) was measured by spectrophotometric method. The results showed significant differences between the two organs of the plant (root and shoot) with respect to the biochemical response tO the applied stress. The cadmium stress caused the accumulation of proline in both shoots and roots. Magnesium reduced the proline content in the samples treated with cadmium. The magnesium treatment seemed to reduce the negative effects of cadmium in wheat seedlings.",
keywords = "Cadmium, Free amino acid, Magnesium, Peroxidase, Polyamine, Proline, Stress, Wheat",
author = "K. Lesk{\'o} and E. Stefanovits-Banyai and L. Simon-Sarkadi",
year = "2002",
language = "English",
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TY - JOUR

T1 - Effect of cadmium and magnesium on free amino acids, polyamines and peroxidase enzyme activity in wheat seedlings

AU - Leskó, K.

AU - Stefanovits-Banyai, E.

AU - Simon-Sarkadi, L.

PY - 2002

Y1 - 2002

N2 - The effect of cadmium and combined cadmium and magnesium was examined on wheat seedlings (Triticum aestivum L. cv. Alföld-90). The one-week-old hydroponically-grown seedlings of wheat were exposed to Cd2+(10-7 M, 10-3 M) and Cd2+(10-7 M, 10-3 M) + 1% MgCl2 stresses. The stress effects were followed by changes in free amino acid, polyamine contents and peroxidase enzyme activity in roots and shoots. Free amino acid content was determined by ion-exchange liquid chromatography. Polyamine content was identified by OPLC (overpressured layer chromatography). Peroxidase activity (U/mg protein) was measured by spectrophotometric method. The results showed significant differences between the two organs of the plant (root and shoot) with respect to the biochemical response tO the applied stress. The cadmium stress caused the accumulation of proline in both shoots and roots. Magnesium reduced the proline content in the samples treated with cadmium. The magnesium treatment seemed to reduce the negative effects of cadmium in wheat seedlings.

AB - The effect of cadmium and combined cadmium and magnesium was examined on wheat seedlings (Triticum aestivum L. cv. Alföld-90). The one-week-old hydroponically-grown seedlings of wheat were exposed to Cd2+(10-7 M, 10-3 M) and Cd2+(10-7 M, 10-3 M) + 1% MgCl2 stresses. The stress effects were followed by changes in free amino acid, polyamine contents and peroxidase enzyme activity in roots and shoots. Free amino acid content was determined by ion-exchange liquid chromatography. Polyamine content was identified by OPLC (overpressured layer chromatography). Peroxidase activity (U/mg protein) was measured by spectrophotometric method. The results showed significant differences between the two organs of the plant (root and shoot) with respect to the biochemical response tO the applied stress. The cadmium stress caused the accumulation of proline in both shoots and roots. Magnesium reduced the proline content in the samples treated with cadmium. The magnesium treatment seemed to reduce the negative effects of cadmium in wheat seedlings.

KW - Cadmium

KW - Free amino acid

KW - Magnesium

KW - Peroxidase

KW - Polyamine

KW - Proline

KW - Stress

KW - Wheat

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