Effect of bacitracin on the biodegradation of β-endorphin

András Patthy, L. Gráf, Ágnes Kenessey, József I. Székely, Sándor Bajusz

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

β-endorphin was incubated with rat brain homogenate, and the amino acids released were measured by amino acid analysis. Phe, Leu, Tyr, and Lys were liberated in the greatest amount indicating that the cleavage of Leu77-Phe78 and some Lys-X peptide bonds with endopeptidases followed by the removal of the terminal residues by exopeptidases are the main routes of β-endorphin degradation in the brain. Bacitracin considerably reduced the amino acid release from β-endorphin incubated with rat brain homogenate, and its action is suggested to be due to the inhibition of brain amino- and carboxypeptidases. Bacitracin also potentiated and prolonged the in vivo analgesic activity of β-endorphin.

Original languageEnglish
Pages (from-to)254-259
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume79
Issue number1
DOIs
Publication statusPublished - Nov 7 1977

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Endorphins
Bacitracin
Biodegradation
Brain
Amino Acids
Rats
Exopeptidases
Carboxypeptidases
Endopeptidases
Aminopeptidases
Analgesics
Degradation
Peptides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effect of bacitracin on the biodegradation of β-endorphin. / Patthy, András; Gráf, L.; Kenessey, Ágnes; Székely, József I.; Bajusz, Sándor.

In: Biochemical and Biophysical Research Communications, Vol. 79, No. 1, 07.11.1977, p. 254-259.

Research output: Contribution to journalArticle

Patthy, András ; Gráf, L. ; Kenessey, Ágnes ; Székely, József I. ; Bajusz, Sándor. / Effect of bacitracin on the biodegradation of β-endorphin. In: Biochemical and Biophysical Research Communications. 1977 ; Vol. 79, No. 1. pp. 254-259.
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