Effect of Asp85 replacement by Thr on the conformation, surface electric properties and stability of bacteriorhodopsin

Stefka G. Taneva, Felix M. Gõi, Nikolai P. Tuparev, Ivana Petkanchin, A. Dér, Arturo Muga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The role of Asp85 in maintaining bacteriorhodopsin structure has been analyzed by infrared spectroscopy, electric light scattering and differential scanning calorimetry. In comparison with the wild type bacteriorhodopsin, the mutant protein D85T shows a different conformation, electric dipole moments and decreased thermal stability. The conformational rearrangements affect both the transmembrane helices and the extramembranous protein segments. Both electric dipoles - the permanent dipole moment and the electric polarizability - have drastically lower values for the membranes containing D85T variant of bacteriorhodopsin. Therefore, this single amino acid mutation not only changes bacteriorhodopsin function, converting the protein into a halorhodopsin-like chloride pump, but it also alters its overall conformation and surface electric state.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume209
Issue number2-3
DOIs
Publication statusPublished - Sep 25 2002

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Bacteriorhodopsins
Conformations
Electric properties
proteins
Proteins
electric dipoles
dipole moments
Electric dipole moments
Halorhodopsins
Dipole moment
electric moments
mutations
Light scattering
helices
amino acids
Amino acids
Differential scanning calorimetry
Infrared spectroscopy
Thermodynamic stability
light scattering

Keywords

  • Bacteriorhodopsin
  • D85T
  • Differential scanning calorimetry
  • Electric light scattering
  • Infrared spectroscopy

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

Effect of Asp85 replacement by Thr on the conformation, surface electric properties and stability of bacteriorhodopsin. / Taneva, Stefka G.; Gõi, Felix M.; Tuparev, Nikolai P.; Petkanchin, Ivana; Dér, A.; Muga, Arturo.

In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 209, No. 2-3, 25.09.2002, p. 193-200.

Research output: Contribution to journalArticle

Taneva, Stefka G. ; Gõi, Felix M. ; Tuparev, Nikolai P. ; Petkanchin, Ivana ; Dér, A. ; Muga, Arturo. / Effect of Asp85 replacement by Thr on the conformation, surface electric properties and stability of bacteriorhodopsin. In: Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2002 ; Vol. 209, No. 2-3. pp. 193-200.
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AU - Muga, Arturo

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