Effect of antimicrobial peptide-amide: Indolicidin on biological membranes

Zsolt Szegletes, Attila Gergely Végh, Krisztina Nagy, Zoltán Bálint, Ádám Kerényi, G. Rákhely, Gyrgy Vár

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Indolicidin, a cationic antimicrobial tridecapeptide amide, is rich in proline and tryptophan residues. Its biological activity is intensively studied, but the details how indolicidin interacts with membranes are not fully understood yet. We report here an in situ atomic force microscopic study describing the effect of indolicidin on an artificial supported planar bilayer membrane of dipalmitoyl phosphatidylcholine (DPPC) and on purple membrane of Halobacterium salinarum. Concentration dependent interaction of the peptide and membranes was found in case of DPPC resulting the destruction of the membrane. Purple membrane was much more resistant against indolicidin, probably due to its high protein content. Indolicidin preferred the border of membrane disks, where the lipids are more accessible. These data suggest that the atomic force microscope is a powerful tool in the study of indolicidin-membrane interaction.

Original languageEnglish
Article number670589
JournalJournal of Biomedicine and Biotechnology
Volume2011
DOIs
Publication statusPublished - 2011

Fingerprint

Biological membranes
Amides
Membranes
Peptides
Purple Membrane
1,2-Dipalmitoylphosphatidylcholine
Halobacterium salinarum
Membrane Lipids
Proline
Tryptophan
indolicidin
Bioactivity
Microscopes
Lipids
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis

Cite this

Effect of antimicrobial peptide-amide : Indolicidin on biological membranes. / Szegletes, Zsolt; Végh, Attila Gergely; Nagy, Krisztina; Bálint, Zoltán; Kerényi, Ádám; Rákhely, G.; Vár, Gyrgy.

In: Journal of Biomedicine and Biotechnology, Vol. 2011, 670589, 2011.

Research output: Contribution to journalArticle

Szegletes, Zsolt ; Végh, Attila Gergely ; Nagy, Krisztina ; Bálint, Zoltán ; Kerényi, Ádám ; Rákhely, G. ; Vár, Gyrgy. / Effect of antimicrobial peptide-amide : Indolicidin on biological membranes. In: Journal of Biomedicine and Biotechnology. 2011 ; Vol. 2011.
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