Effect of antifungal agents on protein composition of Candida albicans studied by capillary electrophoresis and chip technology

Ildikó Kustos, Adrienn Nyul, T. Lóránd, Béla Kocsis, F. Kilár

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In the present study protein profile of a Candida albicans strain had been examined by chip technology and conventional capillary electrophoresis (CE). Profiles could be characterised by the presence of ten dominating protein peaks. These proteins could be distinguished by both techniques, but their quantity showed significant differences in the electropherograms obtained by CE and chip method. Changes in the protein profile were induced by administration of different antifungal agents. Fluconazole and amphotericin B treatment was able to induce similar changes in the pattern, appearance of a 40-kDa protein and up-regulation of a 60-kDa protein was observed by chip technology. Increase in the quantity of these proteins under stress effect (antifungal treatment) might refer to their stress function in the fungal cell. Treatment of C. albicans cells with MK 94 (fused cyclic Mannich ketone) antifungal compound induced not only the previously mentioned changes, but further specific alterations, appearance of a 19-kDa protein and up-regulation of the low molecular weight proteins. This might refer to the different mode of action of this agent on the fungal cells. Conventional capillary electrophoresis was suitable to detect the appearance of the 19-kDa peak, and up-regulation of the 60 kDa protein, but the other changes could not be detected by this technique. Shorter running time, more effective and baseline separation of proteins refer to the advantages of microchip-based method in the analysis of complex biological samples.

Original languageEnglish
Pages (from-to)57-65
Number of pages9
JournalJournal of Biochemical and Biophysical Methods
Volume69
Issue number1-2
DOIs
Publication statusPublished - Nov 30 2006

Fingerprint

Capillary electrophoresis
Candida
Antifungal Agents
Capillary Electrophoresis
Candida albicans
Technology
Chemical analysis
Proteins
Up-Regulation
Fluconazole
Amphotericin B
Heat-Shock Proteins
Ketones
Molecular Weight
Molecular weight

Keywords

  • Antifungal drugs
  • Candida albicans
  • Capillary electrophoresis
  • Fused cyclic Mannich ketone
  • Microchip electrophoresis
  • Stress proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Effect of antifungal agents on protein composition of Candida albicans studied by capillary electrophoresis and chip technology. / Kustos, Ildikó; Nyul, Adrienn; Lóránd, T.; Kocsis, Béla; Kilár, F.

In: Journal of Biochemical and Biophysical Methods, Vol. 69, No. 1-2, 30.11.2006, p. 57-65.

Research output: Contribution to journalArticle

@article{21b7e3c501154b7f964b1918446e6b82,
title = "Effect of antifungal agents on protein composition of Candida albicans studied by capillary electrophoresis and chip technology",
abstract = "In the present study protein profile of a Candida albicans strain had been examined by chip technology and conventional capillary electrophoresis (CE). Profiles could be characterised by the presence of ten dominating protein peaks. These proteins could be distinguished by both techniques, but their quantity showed significant differences in the electropherograms obtained by CE and chip method. Changes in the protein profile were induced by administration of different antifungal agents. Fluconazole and amphotericin B treatment was able to induce similar changes in the pattern, appearance of a 40-kDa protein and up-regulation of a 60-kDa protein was observed by chip technology. Increase in the quantity of these proteins under stress effect (antifungal treatment) might refer to their stress function in the fungal cell. Treatment of C. albicans cells with MK 94 (fused cyclic Mannich ketone) antifungal compound induced not only the previously mentioned changes, but further specific alterations, appearance of a 19-kDa protein and up-regulation of the low molecular weight proteins. This might refer to the different mode of action of this agent on the fungal cells. Conventional capillary electrophoresis was suitable to detect the appearance of the 19-kDa peak, and up-regulation of the 60 kDa protein, but the other changes could not be detected by this technique. Shorter running time, more effective and baseline separation of proteins refer to the advantages of microchip-based method in the analysis of complex biological samples.",
keywords = "Antifungal drugs, Candida albicans, Capillary electrophoresis, Fused cyclic Mannich ketone, Microchip electrophoresis, Stress proteins",
author = "Ildik{\'o} Kustos and Adrienn Nyul and T. L{\'o}r{\'a}nd and B{\'e}la Kocsis and F. Kil{\'a}r",
year = "2006",
month = "11",
day = "30",
doi = "10.1016/j.jbbm.2006.02.002",
language = "English",
volume = "69",
pages = "57--65",
journal = "Journal of Proteomics",
issn = "1874-3919",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Effect of antifungal agents on protein composition of Candida albicans studied by capillary electrophoresis and chip technology

AU - Kustos, Ildikó

AU - Nyul, Adrienn

AU - Lóránd, T.

AU - Kocsis, Béla

AU - Kilár, F.

PY - 2006/11/30

Y1 - 2006/11/30

N2 - In the present study protein profile of a Candida albicans strain had been examined by chip technology and conventional capillary electrophoresis (CE). Profiles could be characterised by the presence of ten dominating protein peaks. These proteins could be distinguished by both techniques, but their quantity showed significant differences in the electropherograms obtained by CE and chip method. Changes in the protein profile were induced by administration of different antifungal agents. Fluconazole and amphotericin B treatment was able to induce similar changes in the pattern, appearance of a 40-kDa protein and up-regulation of a 60-kDa protein was observed by chip technology. Increase in the quantity of these proteins under stress effect (antifungal treatment) might refer to their stress function in the fungal cell. Treatment of C. albicans cells with MK 94 (fused cyclic Mannich ketone) antifungal compound induced not only the previously mentioned changes, but further specific alterations, appearance of a 19-kDa protein and up-regulation of the low molecular weight proteins. This might refer to the different mode of action of this agent on the fungal cells. Conventional capillary electrophoresis was suitable to detect the appearance of the 19-kDa peak, and up-regulation of the 60 kDa protein, but the other changes could not be detected by this technique. Shorter running time, more effective and baseline separation of proteins refer to the advantages of microchip-based method in the analysis of complex biological samples.

AB - In the present study protein profile of a Candida albicans strain had been examined by chip technology and conventional capillary electrophoresis (CE). Profiles could be characterised by the presence of ten dominating protein peaks. These proteins could be distinguished by both techniques, but their quantity showed significant differences in the electropherograms obtained by CE and chip method. Changes in the protein profile were induced by administration of different antifungal agents. Fluconazole and amphotericin B treatment was able to induce similar changes in the pattern, appearance of a 40-kDa protein and up-regulation of a 60-kDa protein was observed by chip technology. Increase in the quantity of these proteins under stress effect (antifungal treatment) might refer to their stress function in the fungal cell. Treatment of C. albicans cells with MK 94 (fused cyclic Mannich ketone) antifungal compound induced not only the previously mentioned changes, but further specific alterations, appearance of a 19-kDa protein and up-regulation of the low molecular weight proteins. This might refer to the different mode of action of this agent on the fungal cells. Conventional capillary electrophoresis was suitable to detect the appearance of the 19-kDa peak, and up-regulation of the 60 kDa protein, but the other changes could not be detected by this technique. Shorter running time, more effective and baseline separation of proteins refer to the advantages of microchip-based method in the analysis of complex biological samples.

KW - Antifungal drugs

KW - Candida albicans

KW - Capillary electrophoresis

KW - Fused cyclic Mannich ketone

KW - Microchip electrophoresis

KW - Stress proteins

UR - http://www.scopus.com/inward/record.url?scp=33749425382&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749425382&partnerID=8YFLogxK

U2 - 10.1016/j.jbbm.2006.02.002

DO - 10.1016/j.jbbm.2006.02.002

M3 - Article

C2 - 16556464

AN - SCOPUS:33749425382

VL - 69

SP - 57

EP - 65

JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

IS - 1-2

ER -